Activation of retinal ganglion cells using a biomimetic artificial retina.

Autor:	Greco JA; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, United States of America., Wagner NL; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, United States of America.; Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, Storrs, CT 06269, United States of America., Jensen RJ; VA Boston Healthcare System, 150 South Huntington Avenue, Boston, MA 02130, United States of America., Lawrence DB; University of Connecticut School of Medicine, 263 Farmington Avenue, Farmington, CT 06032, United States of America., Ranaghan MJ; Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, Storrs, CT 06269, United States of America., Sandberg MN; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, United States of America., Sandberg DJ; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, United States of America., Birge RR; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT 06269, United States of America.; Department of Molecular and Cell Biology, University of Connecticut, 91 North Eagleville Road, Storrs, CT 06269, United States of America.
Jazyk:	angličtina
Zdroj:	Journal of neural engineering [J Neural Eng] 2021 Dec 01; Vol. 18 (6). Date of Electronic Publication: 2021 Dec 01.
DOI:	10.1088/1741-2552/ac395c
Abstrakt:	Objective. Biomimetic protein-based artificial retinas offer a new paradigm for restoring vision for patients blinded by retinal degeneration. Artificial retinas, comprised of an ion-permeable membrane and alternating layers of bacteriorhodopsin (BR) and a polycation binder, are assembled using layer-by-layer electrostatic adsorption. Upon light absorption, the oriented BR layers generate a unidirectional proton gradient. The main objective of this investigation is to demonstrate the ability of the ion-mediated subretinal artificial retina to activate retinal ganglion cells (RGCs) of degenerated retinal tissue. Approach. Ex vivo extracellular recording experiments with P23H line 1 rats are used to measure the response of RGCs following selective stimulation of our artificial retina using a pulsed light source. Single-unit recording is used to evaluate the efficiency and latency of activation, while a multielectrode array (MEA) is used to assess the spatial sensitivity of the artificial retina films. Main results. The activation efficiency of the artificial retina increases with increased incident light intensity and demonstrates an activation latency of ∼150 ms. The results suggest that the implant is most efficient with 200 BR layers and can stimulate the retina using light intensities comparable to indoor ambient light. Results from using an MEA show that activation is limited to the targeted receptive field. Significance. The results of this study establish potential effectiveness of using an ion-mediated artificial retina to restore vision for those with degenerative retinal diseases, including retinitis pigmentosa. (Creative Commons Attribution license.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu