Isolation of Primary Hepatocytes for Testing Tumor Penetrating Peptides.

Autor:	Savier E; Department of Hepatobiliary and Liver Transplantation Surgery, AP-HP, Pitié-Salpêtrière Hospital, Sorbonne Université, Paris, France., Tuffery P; Université de Paris, BFA, UMR 8251, CNRS ERL U1133, Inserm, Paris, France., Bruzzoni-Giovanelli H; Université de Paris, Centre d'Investigation Clinique 1427, Inserm/AP-HP, Saint Louis Hospital, Paris, France., Rebollo A; Université de Paris, Faculté de Pharmacie, UTCBS, Inserm 1265-CNRS UMR8258, Paris, France. angelita.rebollo@parisdescartes.fr.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2383, pp. 413-427.
DOI:	10.1007/978-1-0716-1752-6_26
Abstrakt:	Methods for isolation of human primary cells is an important tool to be able to test the effect of several drugs for the treatment of diseases. Protocols have been described for the isolation of healthy and tumoral hepatocytes; however, the quality and the amount of the isolated cells is not satisfactory. We describe here protocols for the isolation of healthy and tumoral hepatocytes as well as the use of tumor penetrating and interfering peptides as new therapeutics. Peptides are showed to be safe drugs and taking into account recent improvements in peptide administration, biodelivery, and bioavailability. Tumor penetrating peptides fused to an interfering peptide have a great potential to become therapeutic agents. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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