Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses.

Autor: Ninh TT; Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences University of Queensland Brisbane Australia.; Department of Plant Biotechnology, Faculty of Biotechnology Vietnam National University of Agriculture Hanoi Vietnam., Gao W; State Key Laboratory of Cotton Biology, School of Life Science Henan University Kaifeng China., Trusov Y; Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences University of Queensland Brisbane Australia., Zhao JR; State Key Laboratory of Cotton Biology, School of Life Science Henan University Kaifeng China., Long L; State Key Laboratory of Cotton Biology, School of Life Science Henan University Kaifeng China., Song CP; State Key Laboratory of Cotton Biology, School of Life Science Henan University Kaifeng China., Botella JR; Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences University of Queensland Brisbane Australia.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2021 Nov 08; Vol. 5 (11), pp. e359. Date of Electronic Publication: 2021 Nov 08 (Print Publication: 2021).
DOI: 10.1002/pld3.359
Abstrakt: Heterotrimeric G protein Gβ-deficient mutants in rice and maize display constitutive immune responses, whereas Arabidopsis Gβ mutants show impaired defense, suggesting the existence of functional differences between monocots and dicots. Using CRISPR/Cas9, we produced one hemizygous tomato line with a mutated SlGB1 Gβ gene. Homozygous slgb1 knockout mutants exhibit all the hallmarks of autoimmune mutants, including development of necrotic lesions, constitutive expression of defense-related genes, and high endogenous levels of salicylic acid (SA) and reactive oxygen species, resulting in early seedling lethality. Virus-induced silencing of Gβ in cotton reproduced the symptoms observed in tomato mutants, confirming that the autoimmune phenotype is not limited to monocot species but is also shared by dicots. Even though multiple genes involved in SA and ethylene signaling are highly induced by Gβ silencing in tomato and cotton, co-silencing of SA or ethylene signaling components in cotton failed to suppress the lethal phenotype, whereas co-silencing of the oxidative burst oxidase RbohD can repress lethality. Despite the autoimmune response observed in slgb1 mutants, we show that SlGB1 is a positive regulator of the pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) response in tomato. We speculate that the phenotypic differences observed between Arabidopsis and tomato/cotton/rice/maize Gβ knockouts do not necessarily reflect divergences in G protein-mediated defense mechanisms.
Competing Interests: The Authors did not report any conflict of interest.
(© 2021 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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