Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma.

Autor:	Nadolny C; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Zhang X; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Chen Q; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Hashmi SF; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Ali W; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Hemme C; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Ahsan N; COBRE Center for Cancer Research Development, Proteomics Core Facility, Rhode Island Hospital Providence, RI 02903, USA.; Division of Biology and Medicine, Brown University Providence, RI 02903, USA.; Department of Chemistry and Biochemistry, University of Oklahoma 101 Stephenson Parkway, Norman, OK 73019, USA., Chen Y; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA., Deng R; Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island 7 Greenhouse Road, Kingston, RI 02881, USA.
Jazyk:	angličtina
Zdroj:	American journal of cancer research [Am J Cancer Res] 2021 Oct 15; Vol. 11 (10), pp. 4746-4767. Date of Electronic Publication: 2021 Oct 15 (Print Publication: 2021).
Abstrakt:	Ubiquitin specific peptidase-2 (USP2) plays important roles in a myriad of cellular activities through deubiquitinating target proteins and its implications in various diseases, especially cancers, are starting to emerge. Our current understanding on USP2 expression in subjects with hepatocellular carcinoma (HCC) and its roles in the pathogenesis of HCC is limited. In this study, we found that USP2 protein and mRNA levels were significantly dysregulated in HCC tumor (HCC-T) when compared to adjacent non-tumor (HCC-NT) or normal liver tissues from both human and mouse HCC model. Among the USP2 isoforms, USP2b was the predominant isoform in the normal liver and markedly down-regulated in HCC-T tissues in both human and mice. Data from overexpression, chemical inhibition and knockout studies consistently demonstrated that USP2b promoted cell proliferation, colony formation and wound healing in HepG2 and Huh 7 cells. On the other hand, USP2b exhibited proapoptotic and pronecrtotic activities through enhancing bile acid-induced apoptosis and necrosis in both HepG2 and Huh 7 cells. Unbiased proteomic analysis of USP2-knockout (KO) and parental HepG2 cells resulted in identification of USP2-regulated downstream target proteins involved in cell proliferation, apoptosis, and tumorigenesis, including serine/threonine kinase 4 (STK4), epidermal growth factor receptor (EGFR), dipeptidyl peptidase 4 (DPP4) and fatty acid binding protein 1 (FABP1). In conclusion, USP2b expression was dysregulated in subjects with HCC and contributed to the pathogenesis of HCC by promoting cell proliferation and exerting proapoptotic and pronecrotic activities. The findings provide the molecular basis for developing therapies for HCC through modulating USP2b expression or activities. Competing Interests: None. (AJCR Copyright © 2021.)
Databáze:	MEDLINE
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