Epigenetic responses to Phytophthora citrophthora gummosis in citrus.

Autor: Rodrigues da Silva A; Departamento de Biologia, Centro de Genética e Biologia Molecular, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, 45662-900, Brazil., da Costa Silva D; Departamento de Biologia, Centro de Genética e Biologia Molecular, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, 45662-900, Brazil., Dos Santos Pinto KN; Departamento de Ciências Agrárias, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, Bahia, 44380-000, Brazil., Santos Filho HP; Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia, 44380-000, Brazil., Coelho Filho MA; Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia, 44380-000, Brazil., Dos Santos Soares Filho W; Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia, 44380-000, Brazil., Ferreira CF; Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia, 44380-000, Brazil., da Silva Gesteira A; Departamento de Biologia, Centro de Genética e Biologia Molecular, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, 45662-900, Brazil; Embrapa Mandioca e Fruticultura, Cruz das Almas, Bahia, 44380-000, Brazil. Electronic address: abelmon.gesteira@embrapa.br.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2021 Dec; Vol. 313, pp. 111082. Date of Electronic Publication: 2021 Oct 05.
DOI: 10.1016/j.plantsci.2021.111082
Abstrakt: Studies show that DNA methylation is associated with plant immunity but little is known as to how this epigenetic mechanism assists plants in adjusting their responses to biotic stress, especially when interacting with an hemibiotrophic pathogen such as citrus Phytophthora. The aim of the present study was to assess the effects of scion-rootstock interaction on plant resistance to P. citrophthora infection and DNA methylation patterns in 'Pera' sweet orange and 'Tahiti' acid lime grafted onto 'Rangpur' lime and 'Tropical' sunki rootstocks reinoculated with P. citrophthora. Results showed that reinoculated plants of the 'Pera' sweet orange/'Rangpur' lime and 'Tahiti' acid lime/'Tropical' sunki combinations with more and less sensitive varieties to Phytophthora, presented smaller stem lesions and increased frequency of full methylation and hemimethylation rates, compared to inoculated plants. In contrast, 'Tahiti' acid lime/'Rangpur' lime, two highly sensitive varieties, and 'Pera'/'Tropical' sunki, two much less sensitive varieties, showed high increases in the frequency of hemimethylation and non-methylation levels. Results suggest that in citrus, both the scion-rootstock interaction and DNA methylation affect the response to P. citrophthora infection. Reinoculated plants, depending on the combination, showed changes in intracellular hyphae growth through the formation of sets of fibers and crystal accumulation in the periderm, cortex, and phloem. In addition, starch grain concentration was higher in reinoculated plants in comparison to inoculated plants. These findings support the assumption that DNA methylation is a plant defense mechanism and therefore may be exploited to improve the response of plants to the gummosis of P. citrophthora in citrus.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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