Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa).

Autor: Xia YH; Department of Biology, Lund University, Sölvegatan 37, 22362, Lund, Sweden.; Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, 41296, Gothenburg, Sweden., Wang HL; Department of Biology, Lund University, Sölvegatan 37, 22362, Lund, Sweden., Ding BJ; Department of Biology, Lund University, Sölvegatan 37, 22362, Lund, Sweden., Svensson GP; Department of Biology, Lund University, Sölvegatan 37, 22362, Lund, Sweden., Jarl-Sunesson C; Department of Biology, Lund University, Sölvegatan 37, 22362, Lund, Sweden., Cahoon EB; Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Hofvander P; Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 101, 23053, Alnarp, Sweden., Löfstedt C; Department of Biology, Lund University, Sölvegatan 37, 22362, Lund, Sweden. christer.lofstedt@biol.lu.se.
Jazyk: angličtina
Zdroj: Journal of chemical ecology [J Chem Ecol] 2021 Dec; Vol. 47 (12), pp. 950-967. Date of Electronic Publication: 2021 Nov 11.
DOI: 10.1007/s10886-021-01316-4
Abstrakt: Synthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C 12 chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional ∆9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T 2 generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T 4 generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone.
(© 2021. The Author(s).)
Databáze: MEDLINE
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