Structures of Class I and Class II Transcription Complexes Reveal the Molecular Basis of RamA-Dependent Transcription Activation.

Autor: Hao M; Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, 200040, China.; Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission of the People's Republic of China, Shanghai, 200040, China.; Section of Structural Biology, Department of Infectious Diseases, Imperial College London, London, SW7 2AZ, UK., Ye F; Section of Structural Biology, Department of Infectious Diseases, Imperial College London, London, SW7 2AZ, UK., Jovanovic M; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK., Kotta-Loizou I; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK., Xu Q; Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, 200040, China.; Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission of the People's Republic of China, Shanghai, 200040, China., Qin X; Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, 200040, China.; Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission of the People's Republic of China, Shanghai, 200040, China., Buck M; Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK., Zhang X; Section of Structural Biology, Department of Infectious Diseases, Imperial College London, London, SW7 2AZ, UK., Wang M; Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, 200040, China.; Key Laboratory of Clinical Pharmacology of Antibiotics, National Health Commission of the People's Republic of China, Shanghai, 200040, China.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2022 Feb; Vol. 9 (4), pp. e2103669. Date of Electronic Publication: 2021 Nov 10.
DOI: 10.1002/advs.202103669
Abstrakt: Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin-regulating antisense RNA (micF). In bacteria, σ 70 , together with activators, controls the majority of genes by recruiting RNA polymerase (RNAP) to the promoter regions. RNAP and σ 70 form a holoenzyme that recognizes -35 and -10 promoter DNA consensus sites. Many activators bind upstream from the holoenzyme and can be broadly divided into two classes. RamA acts as a class I activator on acrA and class II activator on micF, respectively. The authors present biochemical and structural data on RamA in complex with RNAP-σ 70 at the two promoters and the data reveal the molecular basis for how RamA assembles and interacts with core RNAP and activates transcription that contributes to antibiotic resistance. Further, comparing with CAP/TAP complexes reveals common and activator-specific features in activator binding and uncovers distinct roles of the two C-terminal domains of RNAP α subunit.
(© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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