Naringin prevents cyclophosphamide-induced erythrocytotoxicity in rats by abrogating oxidative stress.

Autor:	Akamo AJ; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Akinloye DI; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Ugbaja RN; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Adeleye OO; Department of Animal Production and Health, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Dosumu OA; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Eteng OE; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Antiya MC; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Amah G; Department of Biochemistry, Benjamin Carson (SRN) School of Medicine, Babcock University, Ilisan, Ogun State, Nigeria., Ajayi OA; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria., Faseun SO; Department of Biochemistry, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Jazyk:	angličtina
Zdroj:	Toxicology reports [Toxicol Rep] 2021 Oct 25; Vol. 8, pp. 1803-1813. Date of Electronic Publication: 2021 Oct 25 (Print Publication: 2021).
DOI:	10.1016/j.toxrep.2021.10.011
Abstrakt:	Earlier reports have shown that Cyclophosphamide (CYCP), an anti-malignant drug, elicited cytotoxicity; and that naringin has several beneficial potentials against oxidative stress and dyslipidaemias. We investigated the influence of naringin on free radical scavenging, cellular integrity, cellular ATP, antioxidants, oxidative stress, and lipid profiles in the CYCP-induced erythrocytotoxicity rat model. Rats were pretreated orally by gavage for fourteen consecutive days with three doses (50, 100, and 200 mg/kg) naringin before single CYCP (200 mg/kg, i.p.) administration. Afterwards, the rats were sacrificed. Naringin concentrations required for 50 % scavenging hydrogen peroxide and nitric oxide radical were 0.27 mg/mL and 0.28 mg/mL, respectively. Naringin pretreatment significantly (p < 0.05) protected erythrocytes plasma membrane architecture and integrity by abolishing CYCP-induced decrease in the activity of erythrocyte LDH (a marker of ATP). Pretreatment with naringin remarkably (p < 0.05) reversed CYCP-induced decreases in the erythrocytes glutathione levels, activities of glutathione-S-transferase, catalase, glutathione peroxidase, and glutathione reductase; attenuated CYCP-mediated increases in erythrocytes levels of malondialdehyde, nitric oxide, and major lipids (cholesterol, triacylglycerol, phospholipids, and non-esterified fatty acids). Taken together, different acute pretreatment doses of naringin might avert CYCP-mediated erythrocytes dysfunctions via its antioxidant, free-radical scavenging, and anti-dyslipidaemia properties. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 Published by Elsevier B.V.)
Databáze:	MEDLINE
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