Preservatives from food-For food: Pea protein hydrolysate as a novel bio-preservative against Escherichia coli O157:H7 on a lettuce leaf.

Autor: Mohan NM; Department of Microbiology Moyne Institute of Preventive Medicine School of Genetics and Microbiology Trinity College Dublin The University of Dublin Dublin Ireland.; Nuritas Limited Dublin Ireland., Zorgani A; Nuritas Limited Dublin Ireland., Earley L; Nuritas Limited Dublin Ireland., Chauhan S; Nuritas Limited Dublin Ireland., Trajkovic S; Nuritas Limited Dublin Ireland., Savage J; Nuritas Limited Dublin Ireland., Adelfio A; Nuritas Limited Dublin Ireland., Khaldi N; Nuritas Limited Dublin Ireland., Martins M; Department of Microbiology Moyne Institute of Preventive Medicine School of Genetics and Microbiology Trinity College Dublin The University of Dublin Dublin Ireland.
Jazyk: angličtina
Zdroj: Food science & nutrition [Food Sci Nutr] 2021 Sep 08; Vol. 9 (11), pp. 5946-5958. Date of Electronic Publication: 2021 Sep 08 (Print Publication: 2021).
DOI: 10.1002/fsn3.2489
Abstrakt: Fresh-cut fruits and vegetables are becoming particularly popular as healthy fast-food options; however, they present challenges such as accelerated rates of decay and increased risk for contamination when compared to whole produce. Given that food safety must remain paramount for producers and manufacturers, research into novel, natural food preservation solutions which can help to ensure food safety and protect against spoilage is on the rise. In this work, we investigated the potential of using a novel protein hydrolysate, produced by the enzymatic hydrolysis of Pisum sativum (PSH), as a novel bio-preservative and its abilities to reduce populations of Escherichia coli O157:H7 after inoculation on a lettuce leaf. While unhydrolyzed P. sativum proteins show no antimicrobial activity, once digested, and purified, the enzymatically released peptides induced in vitro bactericidal effects on the foodborne pathogen at 8 mg/ml. When applied on an infected lettuce leaf, the PSH significantly reduced the number of bacteria recovered after 2 hr of treatment. PSH may be preferred over other preservation strategies based on its natural, inexpensive, sustainable source, environmentally friendly process, nontoxic nature, good batch to batch consistency, and ability to significantly reduce counts of E. coli both in vitro and in a lettuce leaf.
Competing Interests: Zorgani A, Earley L, Chauhan S, Trajkovic S, Savage J, Adelfio A, and Khaldi N were employed by the company Nuritas Limited. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(© 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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