Assessing the microbiota of recycled bedding sand on a Wisconsin dairy farm.

Autor: Pilch HE; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, 53706, USA., Steinberger AJ; Department of Bacteriology, University of Wisconsin-Madison, Madison, 53706, USA.; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, 53706, USA., Sockett DC; Wisconsin Veterinary Diagnostic Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, 53706, USA., Aulik N; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, 53706, USA.; Wisconsin Veterinary Diagnostic Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, 53706, USA., Suen G; Department of Bacteriology, University of Wisconsin-Madison, Madison, 53706, USA., Czuprynski CJ; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, 53706, USA. charles.czuprynski@wisc.edu.
Jazyk: angličtina
Zdroj: Journal of animal science and biotechnology [J Anim Sci Biotechnol] 2021 Nov 11; Vol. 12 (1), pp. 114. Date of Electronic Publication: 2021 Nov 11.
DOI: 10.1186/s40104-021-00635-6
Abstrakt: Background: Sand is often considered the preferred bedding material for dairy cows as it is thought to have lower bacterial counts than organic bedding materials and cows bedded on sand experience fewer cases of lameness and disease. Sand can also be efficiently recycled and reused, making it cost-effective. However, some studies have suggested that the residual organic material present in recycled sand can serve as a reservoir for commensal and pathogenic bacteria, although no studies have yet characterized the total bacterial community composition. Here we sought to characterize the bacterial community composition of a Wisconsin dairy farm bedding sand recycling system and its dynamics across several stages of the recycling process during both summer and winter using 16S rRNA gene amplicon sequencing.
Results: Bacterial community compositions of the sand recycling system differed by both seasons and stage. Summer samples had higher richness and distinct community compositions, relative to winter samples. In both summer and winter samples, the diversity of recycled sand decreased with time drying in the recycling room. Compositionally, summer sand 14 d post-recycling was enriched in operational taxonomic units (OTUs) belonging to the genera Acinetobacter and Pseudomonas, relative to freshly washed sand and sand from cow pens. In contrast, no OTUs were found to be enriched in winter sand. The sand recycling system contained an overall core microbiota of 141 OTUs representing 68.45% ± 10.33% SD of the total bacterial relative abundance at each sampled stage. The 4 most abundant genera in this core microbiota included Acinetobacter, Psychrobacter, Corynebacterium, and Pseudomonas. Acinetobacter was present in greater abundance in summer samples, whereas Psychrobacter and Corynebacterium had higher relative abundances in winter samples. Pseudomonas had consistent relative abundances across both seasons.
Conclusions: These findings highlight the potential of recycled bedding sand as a bacterial reservoir that warrants further study.
(© 2021. The Author(s).)
Databáze: MEDLINE
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