RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis.

Autor: Díaz-Tatis PA; Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá D.C., Colombia.; Grupo de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad Antonio Nariño, Cra1 #47a15, Bogotá D.C., Colombia., Ochoa JC; Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá D.C., Colombia.; Department of Integrative Biology, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479, Poznan, Poland., Rico EM; Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá D.C., Colombia., Rodríguez C; Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá D.C., Colombia.; Ludwig Maximilian University of Munich, Biozentrum Martinsried, Grosshaderner Strasse 4, Martinsried, Germany., Medina A; Transformation Platform, Centro Internacional de Agricultura Tropical (CIAT), Km17 Cali-Palmira, Palmira, Colombia., Szurek B; UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France., Chavarriaga P; Transformation Platform, Centro Internacional de Agricultura Tropical (CIAT), Km17 Cali-Palmira, Palmira, Colombia., López CE; Manihot Biotec, Departamento de Biología, Universidad Nacional de Colombia, Cra30 #45-03, Bogotá D.C., Colombia. celopezc@unal.edu.co.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2022 Jun; Vol. 109 (3), pp. 313-324. Date of Electronic Publication: 2021 Nov 10.
DOI: 10.1007/s11103-021-01211-2
Abstrakt: Key Message: The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms. Cassava (Manihot esculenta) is a tropical root crop affected by different pathogens including Xanthomonas phaseoli pv. manihotis (Xpm), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to Xpm strain CIO151 called RXam2. Transgenic cassava plants overexpressing RXam2 were generated and analyzed. Plants overexpressing RXam2 showed a reduction in bacterial growth to Xpm strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the RXam2 promoter region. The Xpm strain transformed with dTALEs allowed the induction of the RXam2 gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that RXam2 contributes to the understanding of the molecular basis of quantitative disease resistance.
(© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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