High-resolution imaging and identification of biomolecules using Nano-DESI coupled to ion mobility spectrometry.

Autor: Unsihuay D; Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA., Yin R; Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA., Sanchez DM; Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA., Yang M; Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA., Li Y; Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Centre and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA., Sun X; Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Centre and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA., Dey SK; Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Centre and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA., Laskin J; Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. Electronic address: jlaskin@purdue.edu.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2021 Nov 22; Vol. 1186, pp. 339085. Date of Electronic Publication: 2021 Sep 21.
DOI: 10.1016/j.aca.2021.339085
Abstrakt: Simultaneous spatial localization and structural characterization of molecules in complex biological samples currently represents an analytical challenge for mass spectrometry imaging (MSI) techniques. In this study, we describe a novel experimental platform, which substantially expands the capabilities and enhances the depth of chemical information obtained in high spatial resolution MSI experiments performed using nanospray desorption electrospray ionization (nano-DESI). Specifically, we designed and constructed a portable nano-DESI MSI platform and coupled it with a drift tube ion mobility (IM) spectrometer-mass spectrometer. We demonstrate imaging of drift time-separated ions with a high spatial resolution of better than ∼25 μm using uterine tissues on day 4 of pregnancy in mice. Collision cross-section measurements provide unique molecular descriptors of molecules observed in nano-DESI-IM-MSI necessary for their unambiguous identification by comparison with databases. Meanwhile, isomer-specific imaging reveals variations in the isomeric composition across the tissue. Furthermore, IM separation efficiently eliminates isobaric and isomeric interferences originating from solvent peaks, overlapping isotopic peaks of endogenous molecules extracted from the tissue, and products of in-source fragmentation, which is critical to obtaining accurate concentration gradients in the sample using MSI. The structural information provided by the IM separation substantially expands the molecular specificity of high-resolution MSI necessary for unraveling the complexity of biological systems.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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