The miRNome function transitions from regulating developmental genes to transposable elements during pollen maturation.

Autor:	Oliver C; Department of Plant Biology, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala 75007, Sweden., Annacondia ML; Department of Plant Biology, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala 75007, Sweden., Wang Z; Department of Plant Biology, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala 75007, Sweden.; College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.; Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs and Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Nanjing Agricultural University, Nanjing 210095, China., Jullien PE; Institute of Plant Sciences, University of Bern, Bern 3013, Switzerland., Slotkin RK; Donald Danforth Plant Science Center, St. Louis, Missouri 63132, USA.; Division of Biological Sciences, University of Missouri Columbia, Columbia, Missouri 65201, USA., Köhler C; Department of Plant Biology, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala 75007, Sweden.; Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm 14476, Germany., Martinez G; Department of Plant Biology, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala 75007, Sweden.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2022 Feb 03; Vol. 34 (2), pp. 784-801.
DOI:	10.1093/plcell/koab280
Abstrakt:	Animal and plant microRNAs (miRNAs) are essential for the spatio-temporal regulation of development. Together with this role, plant miRNAs have been proposed to target transposable elements (TEs) and stimulate the production of epigenetically active small interfering RNAs. This activity is evident in the plant male gamete containing structure, the male gametophyte or pollen grain. How the dual role of plant miRNAs, regulating both genes and TEs, is integrated during pollen development and which mRNAs are regulated by miRNAs in this cell type at a genome-wide scale are unknown. Here, we provide a detailed analysis of miRNA dynamics and activity during pollen development in Arabidopsis thaliana using small RNA and degradome parallel analysis of RNA end high-throughput sequencing. Furthermore, we uncover miRNAs loaded into the two main active Argonaute (AGO) proteins in the uninuclear and mature pollen grain, AGO1 and AGO5. Our results indicate that the developmental progression from microspore to mature pollen grain is characterized by a transition from miRNAs targeting developmental genes to miRNAs regulating TE activity. (© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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