Effects of lighting patterns in pubertal development and metabolism of female wistar rats.

Autor: Constantino DB; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Electronic address: dconstantino@hcpa.edu.br., Tonon AC; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., de Oliveira MAB; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Amando GR; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Freitas JJ; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Xavier NB; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Ribeiro RJ; Instituto de Informática, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Idiart M; Departamento de Física, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Hidalgo MPL; Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande de Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Cronobiologia e Sono, Hospital de Clínicas de Porto Alegre (HPCA), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Jazyk: angličtina
Zdroj: Physiology & behavior [Physiol Behav] 2022 Jan 01; Vol. 243, pp. 113641. Date of Electronic Publication: 2021 Nov 06.
DOI: 10.1016/j.physbeh.2021.113641
Abstrakt: Modern lifestyle is characterized by constant exposure to artificial light, which is associated with alterations in biological rhythms, abnormalities to reproductive cycles and metabolic changes. In this study, we aimed to evaluate the effects of four different lighting patterns on puberty timing and on possible metabolic changes in female Wistar rats. Additionally, we developed a machine learning algorithm to automatically classify the stages of the estrous cycle. Adult Wistar rats mated during a week at a photoperiod station where they were exposed to combined red-green-blue lights (RGB) during the photoperiod that varied its spectral composition (i.e., variable color temperature) during the day (RGB-v; N = 14), RGB during the photoperiod with a fixed light color temperature (RGB-f; N = 13) during the whole photoperiod; constant darkness (DD; N = 13) and constant fixed light (LL; N = 15). Experiments were performed only on female litters from postnatal day (PND) 22 to 50. Body weight, puberty onset, estrous cyclicity and serum metabolic parameters were measured. We also collected pictures of vaginal smears to create a dataset of 15,936 images to construct an automatic classifier based on convolutional neural networks. No significant differences were found in the age of vaginal opening; however, the RGB-v group showed a significantly lower number of complete and consecutives cycles. Also, the RGB-f group showed the first complete estrous cycle significantly earlier than the RGB-v group. Female rats housed in LL condition presented significantly lower mean body weight from PND 33 to PDN 47 compared to the other groups. Furthermore, higher levels of plasma triglycerides were found in the DD group compared to RGB-f and RGB-v. HDL levels were significantly lower in RGB-v compared to RGB-f and LL groups. Total cholesterol was significantly lower in RGB-v compared to all groups. Visceral fat was significantly higher in RGB-f compared to the LL group. These results suggest that both changes in photoperiod and lighting quality affect pubertal development and alter lipid profiles and visceral fat accumulation.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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