Behavioral, Cellular and Molecular Responses to Cold and Mechanical Stimuli in Rats with Bilateral Dopamine Depletion in the Mesencephalic Dopaminergic Neurons.

Autor: Elshennawy M; Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Université Clermont Auvergne, CHU, CNRS, Clermont Auvergne INP, Institut Pascal, 63000 Clermont-Ferrand, France. Electronic address: menna.faisal@med.asu.edu.eg., Ouachikh O; Université Clermont Auvergne, CHU, CNRS, Clermont Auvergne INP, Institut Pascal, 63000 Clermont-Ferrand, France. Electronic address: omar.ouachikh@uca.fr., Aissouni Y; Université Clermont Auvergne, INSERM, NeuroDol U1107, 63000 Clermont-Ferrand, France. Electronic address: youssef.aissouni@inserm.fr., Youssef S; Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: shahira_youssef@med.asu.edu.eg., Zaki SS; Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: shahira_samir@med.asu.edu.eg., Durif F; Université Clermont Auvergne, CHU, CNRS, Clermont Auvergne INP, Institut Pascal, 63000 Clermont-Ferrand, France. Electronic address: franck.durif@uca.fr., Hafidi A; Université Clermont Auvergne, CHU, CNRS, Clermont Auvergne INP, Institut Pascal, 63000 Clermont-Ferrand, France. Electronic address: aziz.hafidi@uca.fr.
Jazyk: angličtina
Zdroj: Neuroscience [Neuroscience] 2021 Dec 15; Vol. 479, pp. 107-124. Date of Electronic Publication: 2021 Nov 06.
DOI: 10.1016/j.neuroscience.2021.10.026
Abstrakt: Pain is the major non-motor symptom in Parkinson's disease (PD). Preclinical studies have mostly investigated mechanical pain by considering the decrease in a nociceptive threshold. Only a few studies have focused on thermal pain in animal models of PD. Therefore, the goal of this study was to assess the thermal nociceptive behavior of rats subjected to 6-hydroxydopamine (6-OHDA) administration, which constitutes an animal model of PD. Thermal plate investigation demonstrated significant thermal sensitivity to cold temperatures of 10 °C and 15 °C, and not to higher temperatures, in 6-OHDA-lesioned rats when compared with sham. 6-OHDA-lesioned rats also showed cold allodynia as demonstrated by a significant difference in the number of flinches, latency and reaction time to acetone stimulus. Ropinirole administration, a dopamine receptor 2 (D2R) agonist, blocked the acetone-induced cold allodynia in 6-OHDA-lesioned rats. In addition, mechanical hypersensitivity and static allodynia, as demonstrated by a significant difference in the vocalization threshold and pain score respectively, were noticed in 6-OHDA-lesioned rats. Acetone stimulus induced a significant increase in extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) phosphorylation, a pain process molecular marker, in the spinal dorsal horn (SDH), the insular and cingulate cortices in 6-OHDA-lesioned rats when compared to sham. In 6-OHDA-lesioned rats, there was a significant augmentation in the expression of both protein kinase C gamma (PKCγ) and glutamate decarboxylase 67 (GAD67) in the SDH. This highlighted an increase in excitation and a decrease in inhibition in the SDH. Overall, the present study demonstrated a clear cold thermal hypersensitivity, in addition to a mechanical one, in 6-OHDA-lesioned rats.
(Copyright © 2021 IBRO. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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