Rhizoctonia bataticola lectin induces apoptosis and inhibits metastasis in ovarian cancer cells by interacting with CA 125 antigen differentially expressed on ovarian cells.

Autor: Hegde P; Department of Studies in Biochemistry, Karnatak University, Dharwad-580003, Karnatak, India., B R S; Department of Studies in Biochemistry, Karnatak University, Dharwad-580003, Karnatak, India., Ballal S; Department of Studies in Biochemistry, Karnatak University, Dharwad-580003, Karnatak, India., Swamy BM; Department of Studies in Biochemistry, Karnatak University, Dharwad-580003, Karnatak, India., Inamdar SR; Department of Studies in Biochemistry, Karnatak University, Dharwad-580003, Karnatak, India. srinamdar2009@gmail.com.
Jazyk: angličtina
Zdroj: Glycoconjugate journal [Glycoconj J] 2021 Dec; Vol. 38 (6), pp. 669-688. Date of Electronic Publication: 2021 Nov 08.
DOI: 10.1007/s10719-021-10027-7
Abstrakt: A N-glycan specific lectin from Rhizoctonia bataticola [RBL] was shown to induce growth inhibitory and apoptotic effect in human ovarian, colon and leukemic cells but mitogenic effect on normal PBMCs as reported earlier, revealing its clinical potential. RBL has unique specificity for high mannose tri and tetra antennary N-glycans, expressed in ovarian cancer and also recognizes glycans which are part of CA 125 antigen, a well known ovarian cancer marker. Hence, in the present study diagnostic and therapeutic potential of RBL was investigated using human ovarian epithelial cancer SKOV3 and OVCAR3 cells known for differentially expressing CA 125. RBL binds differentially to human ovarian normal, cyst and cancer tissues. Flow cytometry, western blot analysis of membrane proteins showed the competitive binding of RBL and CA 125 antibody for the same binding sites on SKOV3 and OVCAR3 cells. RBL has strong binding to both SKOV3 and OVCAR3 cells with MFI of 173 and 155 respectively. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 2.5 and 8 μg/mL respectively for SKOV3 and OVCAR3 cells. RBL induces reproductive cell death, morphological changes, nuclear degradation and increased release of ROS in SKOV3 and OVCAR3 cells leading to cell death. This is also supported by increase in hypodiploid population, altered MMP leading to apoptosis possibly involving intrinsic pathway. Adhesion, wound healing, invasion and migration assays demonstrated anti-metastasis effect of RBL apart from its growth inhibitory effect. These results show the promising potential of RBL both as a diagnostic and therapeutic agent.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: