Micro/nanoengineered technologies for human pluripotent stem cells maintenance and differentiation.

Autor: Esfahani SN; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Resto Irizarry AM; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Xue X; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Lee SB; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA., Shao Y; Department of Engineering Mechanics, Tsinghua University, Beijing, China., Fu J; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Jazyk: angličtina
Zdroj: Nano today [Nano Today] 2021 Dec; Vol. 41. Date of Electronic Publication: 2021 Oct 06.
DOI: 10.1016/j.nantod.2021.101310
Abstrakt: Human pluripotent stem cells (hPSCs) are a promising source of cells for cell replacement-based therapies as well as modeling human development and diseases in vitro . However, achieving fate control of hPSC with a high yield and specificity remains challenging. The fate specification of hPSCs is regulated by biochemical and biomechanical cues in their environment. Driven by this knowledge, recent exciting advances in micro/nanoengineering have been leveraged to develop a broad range of tools for the generation of extracellular biomechanical and biochemical signals that determine the behavior of hPSCs. In this review, we summarize such micro/nanoengineered technologies for controlling hPSC fate and highlight the role of biochemical and biomechanical cues such as substrate rigidity, surface topography, and cellular confinement in the hPSC-based technologies that are on the horizon.
Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Databáze: MEDLINE
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