Application of dendrimer-based nanosensors in immunodiagnosis.

Autor: Thakare S; Savitribai Phule Pune University, Pune 411007, India., Shaikh A; Nanobioscience, Agharkar Research Institute, Pune 411004, India; Savitribai Phule Pune University, Pune 411007, India., Bodas D; Nanobioscience, Agharkar Research Institute, Pune 411004, India; Savitribai Phule Pune University, Pune 411007, India. Electronic address: dsbodas@aripune.org., Gajbhiye V; Nanobioscience, Agharkar Research Institute, Pune 411004, India; Savitribai Phule Pune University, Pune 411007, India. Electronic address: cme_virendra@yahoo.co.in.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2022 Jan; Vol. 209 (Pt 2), pp. 112174. Date of Electronic Publication: 2021 Oct 24.
DOI: 10.1016/j.colsurfb.2021.112174
Abstrakt: Conventional immunoassays such as ELISA and FLISA have been used for clinical diagnosis for a long time. These assays are complex, time-consuming, and uneconomical. They have been overwhelmed with newer and more efficient methods such as electrochemical and electrochemiluminescent immunosensors that are cost-effective and require less time. Immunosensor is a biosensor that consists of a signal transducer and a biologically interactive system such as antigen and antibody interaction. Recent advances in nanotechnology have seen numerous efforts towards the usage of nanoparticles such as dendrimers in immunoassays. Dendrimers are highly branched structures with a high density of active peripheral groups, expanding their wide range of applications in immunoassays. A vast number of peripheral groups enrich the sensitivity of the immunosensor by governing the orientation of the antibody on the sensor surface. The current review highlights recent progress and developments in applying dendrimers for different immunoassays and their applicability in analyzing various biomarkers in clinical disease diagnosis.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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