A near-infrared AIE fluorescent probe for myelin imaging: From sciatic nerve to the optically cleared brain tissue in 3D.

Autor:	Wu MY; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China.; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China., Wong AYH; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China., Leung JK; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China., Kam C; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China., Wu KL; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, and State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong, China., Chan YS; School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, and State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong, China., Liu K; Division of Life Science, State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Hong Kong, China.; Hong Kong Center for Neurodegenerative Diseases, Hong Kong, China., Ip NY; Division of Life Science, State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Hong Kong, China.; Hong Kong Center for Neurodegenerative Diseases, Hong Kong, China., Chen S; Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Hong Kong, China; sijie.chen@ki.se.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Nov 09; Vol. 118 (45).
DOI:	10.1073/pnas.2106143118
Abstrakt:	Myelin, the structure that surrounds and insulates neuronal axons, is an important component of the central nervous system. The visualization of the myelinated fibers in brain tissues can largely facilitate the diagnosis of myelin-related diseases and understand how the brain functions. However, the most widely used fluorescent probes for myelin visualization, such as Vybrant DiD and FluoroMyelin, have strong background staining, low-staining contrast, and low brightness. These drawbacks may originate from their self-quenching properties and greatly limit their applications in three-dimensional (3D) imaging and myelin tracing. Chemical probes for the fluorescence imaging of myelin in 3D, especially in optically cleared tissue, are highly desirable but rarely reported. We herein developed a near-infrared aggregation-induced emission (AIE)-active probe, PM-ML, for high-performance myelin imaging. PM-ML is plasma membrane targeting with good photostability. It could specifically label myelinated fibers in teased sciatic nerves and mouse brain tissues with a high-signal-to-background ratio. PM-ML could be used for 3D visualization of myelin sheaths, myelinated fibers, and fascicles with high-penetration depth. The staining is compatible with different brain tissue-clearing methods, such as Clear T and Clear T2 The utility of PM-ML staining in demyelinating disease studies was demonstrated using the mouse model of multiple sclerosis. Together, this work provides an important tool for high-quality myelin visualization across scales, which may greatly contribute to the study of myelin-related diseases. Competing Interests: The authors declare no competing interest. (Copyright © 2021 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu