New Insights into Controlling Homoacetogenesis in the Co-digestion of Coffee Waste: Effect of Operational Conditions and Characterization of Microbial Communities.

Autor: Montoya ACV; Laboratory of Biological Processes, Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Campus II, São Carlos, SP, CEP 13563-120, Brazil. acvillamontoya@gmail.com., da Silva Mazareli RC; Laboratory of Biological Processes, Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Campus II, São Carlos, SP, CEP 13563-120, Brazil., Delforno TP; SENAI Innovation Institute for Biotechnology, Bom Retiro, SP, CEP 01130-000, Brazil., Centurion VB; Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), State University of Campinas, Campinas, SP, CEP 13081-970, Brazil., de Oliveira VM; Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), State University of Campinas, Campinas, SP, CEP 13081-970, Brazil., Silva EL; Center of Exact Sciences and Technology, Department of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, CEP 13565-905, Brazil., Varesche MBA; Laboratory of Biological Processes, Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Campus II, São Carlos, SP, CEP 13563-120, Brazil. varesche@sc.usp.br.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2022 Apr; Vol. 194 (4), pp. 1458-1478. Date of Electronic Publication: 2021 Nov 05.
DOI: 10.1007/s12010-021-03725-3
Abstrakt: In this research batch reactors were operated with coffee processing waste and autochthonous microbial consortium, and a taxonomic and functional analysis was performed for phase I of stabilization of maximum H 2 production and for phase II of maximum H 2 consumption. During phase I, the reactor's operating conditions were pH 4.84 to 8.18, headspace 33.18% to 66.82%, and pulp and husk from 6.95 to 17.05 g/L. These assays continued for phase II, with initial pH conditions of 5.8-8.1, headspace of 33.18-66.82%, and pulp and husk remaining from phase I. The highest homoacetogenesis was observed in assay 5 with pH 7.7, 40% headspace, and 15 g/L of pulp and husk (initial concentrations of phase I). A relative abundance of Clostridium 41%, Lactobacillus 20% and Acetobacter 14% was observed in phase I. In phase II, there was a change in relative abundance of 21%, 63%, and 1%, respectively, and functional genes involved with autotrophic (formyltetrahydrofolate synthase) and heterotrophic (enolase) homoacetogenesis, butanol (3-hydroxybutyryl-CoA dehydrogenase), and propionic acid (propionate CoA-transferase) were identified. This study provides a new and amplified insight into the physicochemical and microbiological factors, which can be used to propose adequate operational conditions to maximize the bioenergy production and reduce homoacetogenesis in biological reactors.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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