Novel suction-based in vivo cutaneous DNA transfection platform.

Autor: Lallow EO; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Jhumur NC; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Ahmed I; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Kudchodkar SB; GeneOne Life Science, Seoul, South Korea., Roberts CC; GeneOne Life Science, Seoul, South Korea., Jeong M; GeneOne Life Science, Seoul, South Korea., Melnik JM; Graduate School of Biomedical Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Park SH; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Muthumani K; GeneOne Life Science, Seoul, South Korea., Shan JW; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Zahn JD; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Shreiber DI; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Singer JP; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Park YK; GeneOne Life Science, Seoul, South Korea., Maslow JN; GeneOne Life Science, Seoul, South Korea., Lin H; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2021 Nov 05; Vol. 7 (45), pp. eabj0611. Date of Electronic Publication: 2021 Nov 05.
DOI: 10.1126/sciadv.abj0611
Abstrakt: This work reports a suction-based cutaneous delivery method for in vivo DNA transfection. Following intradermal Mantoux injection of plasmid DNA in a rat model, a moderate negative pressure is applied to the injection site, a technique similar to Chinese báguàn and Middle Eastern hijama cupping therapies. Strong GFP expression was demonstrated with pEGFP-N1 plasmids where fluorescence was observed as early as 1 hour after dosing. Modeling indicates a strong correlation between focal strain/stress and expression patterns. The absence of visible and/or histological tissue injury contrasts with current in vivo transfection systems such as electroporation. Specific utility was demonstrated with a synthetic SARS-CoV-2 DNA vaccine, which generated host humoral immune response in rats with notable antibody production. This method enables an easy-to-use, cost-effective, and highly scalable platform for both laboratorial transfection needs and clinical applications for nucleic acid–based therapeutics and vaccines.
Databáze: MEDLINE