Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements.

Autor: Milavec M; Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia. mojca.milavec@nib.si., Cleveland MH; National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA., Bae YK; Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea., Wielgosz RI; Bureau International Des Poids Et Mesures (BIPM), Pavillon de Breteuil, 92312, Sèvres Cedex, France., Vonsky M; D.I. Mendeleev Institute for Metrology, Moskovsky pr., 19, Saint-Petersburg, 190005, Russian Federation., Huggett JF; National Measurement Laboratory (NML), LGC, Queens Road, Teddington, TW11 0LY, Middlesex, UK.; School of Biosciences & Medicine, Faculty of Health & Medical Science, University of Surrey, Guildford, UK.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2022 Jan; Vol. 414 (2), pp. 791-806. Date of Electronic Publication: 2021 Nov 04.
DOI: 10.1007/s00216-021-03712-x
Abstrakt: Nucleic acid analysis is used in many areas of life sciences such as medicine, food safety, and environmental monitoring. Accurate, reliable measurements of nucleic acids are crucial for maximum impact, yet users are often unaware of the global metrological infrastructure that exists to support these measurements. In this work, we describe international efforts to improve nucleic acid analysis, with a focus on the Nucleic Acid Analysis Working Group (NAWG) of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology (CCQM). The NAWG is an international group dedicated to improving the global comparability of nucleic acid measurements; its primary focus is to support the development and maintenance of measurement capabilities and the dissemination of measurement services from its members: the National Metrology Institutes (NMIs) and Designated Institutes (DIs). These NMIs and DIs provide DNA and RNA measurement services developed in response to the needs of their stakeholders. The NAWG members have conducted cutting edge work over the last 20 years, demonstrating the ability to support the reliability, comparability, and traceability of nucleic acid measurement results in a variety of sectors.
(© 2021. The Author(s).)
Databáze: MEDLINE
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