Evaluation of ATAD2 as a Potential Target in Hepatocellular Carcinoma.

Autor: Ekin U; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Turkey., Yuzugullu H; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.; Institut Albert Bonniot, Grenoble, France., Ozen C; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.; Present Address: Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Bioinformatics group, Dresden, Germany., Korhan P; Izmir Biomedicine and Genome Center, Izmir, Turkey., Bagirsakci E; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Turkey., Yilmaz F; Department of Pathology, Faculty of Medicine, Ege University, Izmir, Turkey., Yuzugullu OG; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.; Institut Albert Bonniot, Grenoble, France., Uzuner H; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Turkey., Alotaibi H; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Turkey., Kirmizibayrak PB; Department of Biochemistry, Faculty of Pharmacy, Ege University, Izmir, Turkey., Atabey N; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Faculty of Medicine, Izmir Tinaztepe University, Izmir, Turkey., Karakülah G; Izmir Biomedicine and Genome Center, Izmir, Turkey.; Izmir International Biomedicine and Genome Institute, Dokuz Eylul University, Izmir, Turkey., Ozturk M; Izmir Biomedicine and Genome Center, Izmir, Turkey. mehmet.ozturk@ibg.edu.tr.; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey. mehmet.ozturk@ibg.edu.tr.; Institut Albert Bonniot, Grenoble, France. mehmet.ozturk@ibg.edu.tr.; Faculty of Medicine, Izmir Tinaztepe University, Izmir, Turkey. mehmet.ozturk@ibg.edu.tr.
Jazyk: angličtina
Zdroj: Journal of gastrointestinal cancer [J Gastrointest Cancer] 2021 Dec; Vol. 52 (4), pp. 1356-1369. Date of Electronic Publication: 2021 Nov 05.
DOI: 10.1007/s12029-021-00732-9
Abstrakt: Purpose: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide with lack of effective systemic chemotherapy. In this study, we aimed to evaluate the value of ATPase family AAA domain-containing protein 2 (ATAD2) as a biomarker and potential therapeutic target for HCC.
Methods: The expression of ATAD2 was tested in different HCC patient cohorts by immunohistochemistry and comparative transcriptional analysis. The co-expression of ATAD2 and proliferation markers was compared during liver regeneration and malignancy with different bioinformatics tools. The cellular effects of ATAD2 inactivation in liver malignancy was tested on cell cycle, apoptosis, and colony formation ability as well as tumor formation using RNA interference. The genes affected by ATAD2 inactivation in three different HCC cell lines were identified by global gene expression profiling and bioinformatics tools.
Results: ATAD2 overexpression is closely correlated with HCC tumor stage. There was gradual increase from dysplasia, well-differentiated and poorly-differentiated HCC, respectively. We also observed transient upregulation of ATAD2 expression during rat liver regeneration in parallel to changes in Ki-67 expression. ATAD2 knockdown resulted in apoptosis and decreased cell survival in vitro and decreased tumor formation in some HCC cell lines. However, three other HCC cell lines tested were not affected. Similarly, gene expression response to ATAD2 inactivation in different HCC cell lines was highly heterogeneous.
Conclusions: ATAD2 is a potential proliferation marker for liver regeneration and HCC. It may also serve as a therapeutic target despite heterogeneous response of malignant cells.
(© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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