Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand.

Autor: Kueakulpattana N; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, King, Thailand.; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Wannigama DL; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, King, Thailand.; School of Medicine, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, WA, Australia.; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Luk-In S; Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand., Hongsing P; Mae Fah Luang University Hospital, Mae Fah Luang University, Chiang Rai, 57100, Thailand.; School of Integrative Medicine, Mae Fah Luang University, Chiang Rai, 57100, Thailand., Hurst C; Department of Statistic, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia., Badavath VN; Institute for Drug Research, The Hebrew University, Jerusalem, 9112001, Israel.; Chitkara College of Pharmacy, Chitkara University, Punjab, 140401, India., Jenjaroenpun P; Division of Bioinformatics and Data Management for Research, Department of Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand., Wongsurawat T; Division of Bioinformatics and Data Management for Research, Department of Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand., Teeratakulpisan N; The Thai Red Cross AIDS Research Centre, Bangkok, Thailand., Kerr SJ; HIV-NAT, Thai Red Cross AIDS Research Centre, Bangkok, Thailand.; Center of Excellence in Biostatistics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Abe S; Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan., Phattharapornjaroen P; Department of Emergency Medicine, Center of Excellence, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand., Shein AMS; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, King, Thailand.; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Saethang T; Department of Computer Science, Faculty of Science, Kasetsart University, Bangkok, Thailand., Chantaravisoot N; Office of Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.; Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Amarasiri M; Laboratory of Environmental HygieneDepartment of Health Science, School of Allied Health Sciences, Kitasato University, Sagamihara-Minami, KitasatoKanagawa, 252-0373, Japan., Higgins PG; Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany.; German Centre for Infection Research, Partner Site Bonn-Cologne, Cologne, Germany., Chatsuwan T; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, King, Thailand. Tanittha.C@chula.ac.th.; Antimicrobial Resistance and Stewardship Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Tanittha.C@chula.ac.th.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Nov 04; Vol. 11 (1), pp. 21659. Date of Electronic Publication: 2021 Nov 04.
DOI: 10.1038/s41598-021-00675-y
Abstrakt: The global rapid emergence of azithromycin/ceftriaxone resistant Neisseria gonorrhoeae threatens current recommend azithromycin/ceftriaxone dual therapy for gonorrhea to ensure effective treatment. Here, we identified the first two N. gonorrhoeae isolates with decreased ceftriaxone susceptibility in Thailand. Among 134 N. gonorrhoeae isolates collected from Thai Red Cross Anonymous Clinic, Bangkok, two isolates (NG-083 and NG-091) from urethral swab in male heterosexual patients had reduced susceptibility to ceftriaxone (MICs of 0.125 mg/L). Both were multidrug resistant and strong biofilm producers with ceftriaxone tolerance (MBEC > 128 mg/L). NG-083 and NG-091 remained susceptible to azithromycin (MIC of 1 mg/L and 0.5 mg/L, respectively). Reduced susceptibility to ceftriaxone was associated with alterations in PBP2, PBP1, PorB, MtrR, and mtrR promoter region. NG-083 belonged to sequence type (ST) 7235 and NG-091 has new allele number of tbpB with new ST. Molecular docking revealed ceftriaxone weakly occupied the active site of mosaic XXXIV penicillin-binding protein 2 variant in both isolates. Molecular epidemiology results revealed that both isolates display similarities with isolates from UK, USA, and The Netherlands. These first two genetically related gonococcal isolates with decreased ceftriaxone susceptibility heralds the threat of treatment failure in Thailand, and importance of careful surveillance.
(© 2021. The Author(s).)
Databáze: MEDLINE
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