Inhibition of the JAZ1 gene causes activation of camalexin biosynthesis in Arabidopsis callus cultures.

Autor: Makhazen DS; Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia. Electronic address: makhazen@biosoil.ru., Veremeichik GN; Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia., Shkryl YN; Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia., Tchernoded GK; Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia., Grigorchuk VP; Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia., Bulgakov VP; Federal Scientific Center of the East Asia Terrestrial Biodiversity of the Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia.
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2021 Dec 10; Vol. 342, pp. 102-113. Date of Electronic Publication: 2021 Nov 01.
DOI: 10.1016/j.jbiotec.2021.10.012
Abstrakt: Indole alkaloid camalexin has potential medicinal properties such as suppressing the viability of leukemic but not normal cells. Camalexin is not produced in plants and an external factor is required to activate its biosynthesis. In this work, we stimulated camalexin biosynthesis in Arabidopsis calli by blocking one of repressors of the jasmonate pathway, the jasmonate ZIM-domain protein 1 (JAZ1) by using amiRNA targeting JAZ1 gene transcripts. Inhibition of the JAZ1 gene led to an increase in camalexin content from trace amounts in control culture to 9 µg/g DW in the jaz1 line without affecting growth. In addition, JAZ1 silencing enhanced tolerance to cold stress with simultaneous increasing camalexin content up to 30 µg/g DW. Real-time quantitative PCR determination of marker gene expression showed that effects caused by the JAZ1 silencing might be realized through crosslinking JA, ROS, and abscisic acid signaling pathways. Thus, targeting the distal components of signaling pathways can be suggested as a tool for bioengineering of secondary metabolism, along with standard techniques for targeting biosynthetic genes or genes encoding transcription factors.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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