Dual function of HPF1 in the modulation of PARP1 and PARP2 activities.

Autor: Kurgina TA; Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia., Moor NA; Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia., Kutuzov MM; Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia., Naumenko KN; Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia., Ukraintsev AA; Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia., Lavrik OI; Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia. lavrik@niboch.nsc.ru.; Novosibirsk State University, Novosibirsk, Russia. lavrik@niboch.nsc.ru.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2021 Nov 03; Vol. 4 (1), pp. 1259. Date of Electronic Publication: 2021 Nov 03.
DOI: 10.1038/s42003-021-02780-0
Abstrakt: Poly(ADP-ribosyl)ation catalyzed by poly(ADP-ribose) polymerases (PARPs) is one of the immediate cellular responses to DNA damage. The histone PARylation factor 1 (HPF1) discovered recently to form a joint active site with PARP1 and PARP2 was shown to limit the PARylation activity of PARPs and stimulate their NAD + -hydrolase activity. Here we demonstrate that HPF1 can stimulate the DNA-dependent and DNA-independent autoPARylation of PARP1 and PARP2 as well as the heteroPARylation of histones in the complex with nucleosome. The stimulatory action is detected in a defined range of HPF1 and NAD + concentrations at which no HPF1-dependent enhancement in the hydrolytic NAD + consumption occurs. PARP2, comparing with PARP1, is more efficiently stimulated by HPF1 in the autoPARylation reaction and is more active in the heteroPARylation of histones than in the automodification, suggesting a specific role of PARP2 in the ADP-ribosylation-dependent modulation of chromatin structure. Possible role of the dual function of HPF1 in the maintaining PARP activity is discussed.
(© 2021. The Author(s).)
Databáze: MEDLINE
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