Targeting IFN activity to both B cells and plasmacytoid dendritic cells induces a robust tolerogenic response and protection against EAE.

Autor: Cauwels A; Cytokine Receptor Laboratory, VIB Medical Biotechnology Center, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium. acauwels@orionisbio.com.; Orionis Biosciences, 9052, Ghent, Belgium. acauwels@orionisbio.com., Van Lint S; Cytokine Receptor Laboratory, VIB Medical Biotechnology Center, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium., Rogge E; Cytokine Receptor Laboratory, VIB Medical Biotechnology Center, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium.; Orionis Biosciences, 9052, Ghent, Belgium., Verhee A; Cytokine Receptor Laboratory, VIB Medical Biotechnology Center, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium., Van Den Eeckhout B; Cytokine Receptor Laboratory, VIB Medical Biotechnology Center, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium., Pang S; Institute of Neuropathology, Faculty of Medicine, University of Freiburg, 79106, Freiburg, Germany., Prinz M; Institute of Neuropathology, Faculty of Medicine, University of Freiburg, 79106, Freiburg, Germany.; BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79106, Freiburg, Germany., Kley N; Orionis Biosciences, 9052, Ghent, Belgium., Uzé G; CNRS UMR 5235, University Montpellier, 34095, Montpellier, France., Tavernier J; Cytokine Receptor Laboratory, VIB Medical Biotechnology Center, Ghent University, A. Baertsoenkaai 3, 9000, Ghent, Belgium. jtavernier@orionisbio.com.; Orionis Biosciences, 9052, Ghent, Belgium. jtavernier@orionisbio.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Nov 03; Vol. 11 (1), pp. 21575. Date of Electronic Publication: 2021 Nov 03.
DOI: 10.1038/s41598-021-00891-6
Abstrakt: Type I Interferon (IFN) was the very first drug approved for the treatment of Multiple Sclerosis (MS), and is still frequently used as a first line therapy. However, systemic IFN also causes considerable side effects, affecting therapy adherence and dose escalation. In addition, the mechanism of action of IFN in MS is multifactorial and still not completely understood. Using AcTaferons (Activity-on-Target IFNs, AFNs), optimized IFN-based immunocytokines that allow cell-specific targeting, we have previously demonstrated that specific targeting of IFN activity to dendritic cells (DCs) can protect against experimental autoimmune encephalitis (EAE), inducing in vivo tolerogenic protective effects, evidenced by increased indoleamine-2,3-dioxygenase (IDO) and transforming growth factor β (TGFβ) release by plasmacytoid (p) DCs and improved immunosuppressive capacity of regulatory T and B cells. We here report that targeting type I IFN activity specifically towards B cells also provides strong protection against EAE, and that targeting pDCs using SiglecH-AFN can significantly add to this protective effect. The superior protection achieved by simultaneous targeting of both B lymphocytes and pDCs correlated with improved IL-10 responses in B cells and conventional cDCs, and with a previously unseen very robust IDO response in several cells, including all B and T lymphocytes, cDC1 and cDC2.
(© 2021. The Author(s).)
Databáze: MEDLINE
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