NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration.

Autor: Laplace-Builhé B; IRMB, Univ Montpellier, INSERM, Montpellier, France., Barthelaix A; IRMB, Univ Montpellier, INSERM, Montpellier, France., Assou S; IRMB, Univ Montpellier, INSERM, Montpellier, France., Bohaud C; IRMB, Univ Montpellier, INSERM, Montpellier, France., Pratlong M; MGX, BCM, Univ Montpellier, CNRS, INSERM, Montpellier, France., Severac D; MGX, BCM, Univ Montpellier, CNRS, INSERM, Montpellier, France., Tejedor G; IRMB, Univ Montpellier, INSERM, Montpellier, France., Luz-Crawford P; Laboratorio de Inmunología Celular y Molecular, Facultad de Medicina, Universidad de los Andes, Santiago, Chile.; IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, Chile., Nguyen-Chi M; LPHI, Univ Montpellier, CNRS, Montpellier, France., Mathieu M; IRMB, Univ Montpellier, INSERM, Montpellier, France., Jorgensen C; IRMB, Univ Montpellier, INSERM, Montpellier, France.; CHU Montpellier, Montpellier, France., Djouad F; IRMB, Univ Montpellier, INSERM, Montpellier, France. farida.djouad@inserm.fr.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Nov 03; Vol. 12 (1), pp. 6336. Date of Electronic Publication: 2021 Nov 03.
DOI: 10.1038/s41467-021-26422-5
Abstrakt: Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.
(© 2021. The Author(s).)
Databáze: MEDLINE
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