| Autor: |
Timoshenko OS; Institute of Biomedical Chemistry, Moscow, Russia., Khmeleva SA; Institute of Biomedical Chemistry, Moscow, Russia., Poverennaya EV; Institute of Biomedical Chemistry, Moscow, Russia., Kiseleva YY; Russian Scientific Center of Roentgenoradiology, Moscow, Russia., Kurbatov LK; Institute of Biomedical Chemistry, Moscow, Russia., Radko SP; Institute of Biomedical Chemistry, Moscow, Russia., Buromski IV; Pirogov Russian National Research Medical University, Moscow, Russia., Markin SS; Institute of Biomedical Chemistry, Moscow, Russia., Lisitsa AV; Institute of Biomedical Chemistry, Moscow, Russia., Archakov AI; Institute of Biomedical Chemistry, Moscow, Russia., Ponomarenko EA; Institute of Biomedical Chemistry, Moscow, Russia. |
| Abstrakt: |
Using human chromosome 18 (Ch18) genes as an example, a PCR analysis of the interindividual variability of gene expression in liver tissue was performed. Although the quantitative profiles of the Ch18 transcriptome, expressed in the number of cDNA copies per single cell, showed a high degree of correlation between donors (Pearson correlation coefficients ranged from 0.963 to 0.966), the expression of the significant number of genes (from 13% to 19%, depending on the method of experimental data normalization) varied by more than 4-fold when comparing donors pairwise. At the same time, the proportion of differentially expressed genes increased with a decrease in the level of their expression. It is shown that the higher quantitative variability of low-abundance transcripts is mainly not technical, but biological. Bioinformatic analysis of the interindividual variability of the differential expression of chromosome 18 genes in human liver tissue did not reveal any statistically significant groups of genes related to certain biological processes that indicated a rather transient nature of the interindividual variability of their expression, probably reflecting the response of cells of an individual to specific external stimuli. |
