Immunoinformatics based designing a multi-epitope vaccine against pathogenic Chandipura vesiculovirus.

Autor: Deb D; Department of Life Sciences, Garden City University, Bangalore, Karnataka, India., Basak S; Department of Life Sciences, Garden City University, Bangalore, Karnataka, India., Kar T; Department of Life Sciences, Garden City University, Bangalore, Karnataka, India., Narsaria U; Department of Life Sciences, Garden City University, Bangalore, Karnataka, India., Castiglione F; Institute for Applied Computing, National Research Council of Italy, Via dei Taurini, Rome, Italy., Paul A; Department of Life Sciences, Garden City University, Bangalore, Karnataka, India., Pandey A; Plant Metabolic Engineering, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India., Srivastava AP; Department of Life Sciences, Garden City University, Bangalore, Karnataka, India.
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2022 Feb; Vol. 123 (2), pp. 322-346. Date of Electronic Publication: 2021 Nov 03.
DOI: 10.1002/jcb.30170
Abstrakt: Chandipura vesiculovirus (CHPV) is a rapidly emerging pathogen responsible for causing acute encephalitis. Due to its widespread occurrence in Asian and African countries, this has become a global threat, and there is an urgent need to design an effective and nonallergenic vaccine against this pathogen. The present study aimed to develop a multi-epitope vaccine using an immunoinformatics approach. The conventional method of vaccine design involves large proteins or whole organism which leads to unnecessary antigenic load with increased chances of allergenic reactions. In addition, the process is also very time-consuming and labor-intensive. These limitations can be overcome by peptide-based vaccines comprising short immunogenic peptide fragments that can elicit highly targeted immune responses, avoiding the chances of allergenic reactions, in a relatively shorter time span. The multi-epitope vaccine constructed using CTL, HTL, and IFN-γ epitopes was able to elicit specific immune responses when exposed to the pathogen, in silico. Not only that, molecular docking and molecular dynamics simulation studies confirmed a stable interaction of the vaccine with the immune receptors. Several physicochemical analyses of the designed vaccine candidate confirmed it to be highly immunogenic and nonallergic. The computer-aided analysis performed in this study suggests that the designed multi-epitope vaccine can elicit specific immune responses and can be a potential candidate against CHPV.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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