Specific Activities of Hydromedusan Ca 2+ -Regulated Photoproteins.

Autor: Malikova NP; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Eremeeva EV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia., Gulnov DV; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia., Natashin PV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Nemtseva EV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia., Vysotski ES; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2022 Jan; Vol. 98 (1), pp. 275-283. Date of Electronic Publication: 2021 Nov 15.
DOI: 10.1111/php.13556
Abstrakt: Nowadays the recombinant Ca 2+ -regulated photoproteins originating from marine luminous organisms are widely applied to monitor calcium transients in living cells due to their ability to emit light on Ca 2+ binding. Here we report the specific activities of the recombinant Ca 2+ -regulated photoproteins-aequorin from Aequorea victoria, obelins from Obelia longissima and Obelia geniculata, clytin from Clytia gregaria and mitrocomin from Mitrocoma cellularia. We demonstrate that along with bioluminescence spectra, kinetics of light signals and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of bioluminescent reactions. The highest specific activities were found for obelins and mitrocomin, whereas those of aequorin and clytin were shown to be lower. To determine the factors influencing the variations in specific activities the fluorescence quantum yields for Ca 2+ -discharged photoproteins were measured and found to be quite different varying in the range of 0.16-0.36. We propose that distinctions in specific activities may result from different efficiencies of singlet excited state generation and different fluorescence quantum yields of coelenteramide bound within substrate-binding cavity. This in turn may be conditioned by variations in the amino acid environment of the substrate-binding cavities and hydrogen bond distances between key residues and atoms of 2-hydroperoxycoelenterazine.
(© 2021 American Society for Photobiology.)
Databáze: MEDLINE
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