[The in vitro effect of antiseptics on epithelial cells of human cornea and conjunctiva].

Autor:	Alexander-Sinclair EI; Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia., Okolov IN; Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia., Perepletchikova DA; Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.; Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia., Grechanaya YS; Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.; Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia., Zhurenkov KE; Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.; Saint Petersburg State University, Saint Petersburg, Russia., Blinova MI; Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia.
Jazyk:	ruština
Zdroj:	Vestnik oftalmologii [Vestn Oftalmol] 2021; Vol. 137 (5), pp. 86-92.
DOI:	10.17116/oftalma202113705186
Abstrakt:	Effective and safe antiseptic eye preparations are necessary for prevention and treatment of infectious and inflammatory eye diseases. Purpose: in vitro evaluation of the effect of antiseptic eye drops on corneal and conjunctival epithelial cells. Material and Methods: Antiseptic eye drops «Bactavit», «Vitabact» and «Ocomistin» were the object of the study. Immortalized human corneal epithelial cell lines (HCE) and human conjunctiva (Chang Conjunctiva, Clone 1-5c-4) were used as the test systems. The viability of the cells was assessed by their metabolic activity and morphology using the MTT test and phase-contrast microscopy. Results: Antiseptic eye drops belonging to different groups of chemical compounds induced cytotoxic effects on the cells of corneal epithelium (HCE) and human conjunctiva (Chang Conjunctiva, Clone 1-5c-4) of varying degrees, leading to morphological and functional changes in those cells. Conclusion: The study confirms the possibility of using cultured cells for the in vitro comparative assessment of the cytotoxic effect of antiseptic ophthalmic agents.
Databáze:	MEDLINE
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