Construction of a lncRNA-miRNA-mRNA network to determine the key regulators of the Th1/Th2 imbalance in multiple sclerosis.

Autor: Azari H; Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, 79196-93116, Iran., Karimi E; Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, 79196-93116, Iran., Shekari M; Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, 79196-93116, Iran.; Hormozgan University of Medical Sciences Research Center for Molecular Medicine, Bandar Abbas, 79196-93116, Iran., Tahmasebi A; Institute of Biotechnology, Shiraz University, Shiraz, 71441-13131, Iran., Nikpoor AR; Hormozgan University of Medical Sciences Research Center for Molecular Medicine, Bandar Abbas, 79196-93116, Iran., Negahi AA; Department of Internal Medicine, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, 79196-93116, Iran., Sanadgol N; Institute of Neuroanatomy, RWTH University Hospital Aachen, Aachen, 52074, Germany., Mousavi P; Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, 79196-93116, Iran.; Hormozgan University of Medical Sciences Research Center for Molecular Medicine, Bandar Abbas, 79196-93116, Iran.
Jazyk: angličtina
Zdroj: Epigenomics [Epigenomics] 2021 Nov; Vol. 13 (22), pp. 1797-1815. Date of Electronic Publication: 2021 Nov 02.
DOI: 10.2217/epi-2021-0296
Abstrakt: Aim: The exact epigenetic mechanisms that determine the balance of T helper (Th)1 and Th2 cells and autoimmune responses in multiple sclerosis (MS) remain unclear. We aim to clarify these. Methods: A combination of bioinformatics analysis and molecular evaluations was utilized to identify master hub genes. Results: A competitive endogenous RNA network containing six long noncoding RNAs (lncRNAs), 21 miRNAs and 86 mRNAs was provided through enrichment analysis and a protein-protein interaction network. NEAT1 and MALAT1 were found as differentially expressed lncRNAs using Gene Expression Omnibus (GSE21942). Quantitative real-time PCR results demonstrate dysregulation in the RUNX3 (a regulator of Th1/Th2 balance), GATA3  and TBX21 , as well as miR-544a and miR-210-3p (which directly target RUNX3 ). ELISA also confirmed an imbalance in IFN-γ (Th1)/IL-4 (Th2) in MS patients. Conclusion: Our findings introduce novel biomarkers leading to Th1/Th2 imbalance in MS.
Databáze: MEDLINE
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