Practical approach to detection and surveillance of emerging highly resistant Mycobacterium tuberculosis Beijing 1071-32-cluster.

Autor: Mokrousov I; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg, Russia, 197101. igormokrousov@yahoo.com., Vyazovaya A; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg, Russia, 197101., Sinkov V; Scientific Centre of the Family Health and Human Reproduction Problems, Irkutsk, Russia., Gerasimova A; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg, Russia, 197101., Ioannidis P; National Reference Laboratory for Mycobacteria, Sotiria Chest Diseases Hospital, Athens, Greece., Jiao W; National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Disease, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China., Khromova P; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg, Russia, 197101.; Scientific Centre of the Family Health and Human Reproduction Problems, Irkutsk, Russia., Papaventsis D; National Reference Laboratory for Mycobacteria, Sotiria Chest Diseases Hospital, Athens, Greece., Pasechnik O; Omsk State Medical University, Omsk, Russia., Perdigão J; Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Lisbon, Portugal., Rastogi N; WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de la Guadeloupe, Abymes, Guadeloupe, France., Shen A; National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Disease, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.; Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, China., Skiba Y; Almaty Branch of National Center for Biotechnology in Central Reference Laboratory, Almaty, Kazakhstan., Solovieva N; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg, Russia., Suffys P; Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Tafaj S; University Hospital 'Shefqet Ndroqi', Tirana, Albania., Umpeleva T; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg, Russia, 197101.; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia., Vakhrusheva D; Ural Research Institute for Phthisiopulmonology, National Medical Research Center of Tuberculosis and Infectious Diseases of Ministry of Health of the Russian Federation, Yekaterinburg, Russia., Yarusova I; St. Petersburg Pasteur Institute, 14 Mira Street, St. Petersburg, Russia, 197101.; Clinical Tuberculosis Dispensary, Omsk, Russia., Zhdanova S; Scientific Centre of the Family Health and Human Reproduction Problems, Irkutsk, Russia., Zhuravlev V; St. Petersburg Research Institute of Phthisiopulmonology, St. Petersburg, Russia., Ogarkov O; Scientific Centre of the Family Health and Human Reproduction Problems, Irkutsk, Russia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Nov 01; Vol. 11 (1), pp. 21392. Date of Electronic Publication: 2021 Nov 01.
DOI: 10.1038/s41598-021-00890-7
Abstrakt: Ancient sublineage of the Mycobacterium tuberculosis Beijing genotype is endemic and prevalent in East Asia and rare in other world regions. While these strains are mainly drug susceptible, we recently identified a novel clonal group Beijing 1071-32 within this sublineage emerging in Siberia, Russia and present in other Russian regions. This cluster included only multi/extensive drug resistant (MDR/XDR) isolates. Based on the phylogenetic analysis of the available WGS data, we identified three synonymous SNPs in the genes Rv0144, Rv0373c, and Rv0334 that were specific for the Beijing 1071-32-cluster and developed a real-time PCR assay for their detection. Analysis of the 2375 genetically diverse M. tuberculosis isolates collected between 1996 and 2020 in different locations (European and Asian parts of Russia, former Soviet Union countries, Albania, Greece, China, Vietnam, Japan and Brazil), confirmed 100% specificity and sensitivity of this real-time PCR assay. Moreover, the epidemiological importance of this strain and the newly developed screening assay is further stressed by the fact that all identified Beijing 1071-32 isolates were found to exhibit MDR genotypic profiles with concomitant resistance to additional first-line drugs due to a characteristic signature of six mutations in rpoB450, rpoC485, katG315, katG335, rpsL43 and embB497. In conclusion, this study provides a set of three concordant SNPs for the detection and screening of Beijing 1071-32 isolates along with a validated real-time PCR assay easily deployable across multiple settings for the epidemiological tracking of this significant MDR cluster.
(© 2021. The Author(s).)
Databáze: MEDLINE
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