Potential Probiotic Pediococcus pentosaceus M41 Modulates Its Proteome Differentially for Tolerances Against Heat, Cold, Acid, and Bile Stresses.

Autor:	Baig MA; Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab Emirates., Turner MS; School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, Australia., Liu SQ; Department of Food Science and Technology, Faculty of Science, National University of Singapore, Singapore, Singapore., Al-Nabulsi AA; Department of Nutrition and Food Technology, Jordan University of Science and Technology, Irbid, Jordan., Shah NP; Food and Nutritional Science, School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, SAR China., Ayyash MM; Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab Emirates.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Oct 13; Vol. 12, pp. 731410. Date of Electronic Publication: 2021 Oct 13 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.731410
Abstrakt:	Probiotics containing functional food confer health benefits in addition to their nutritional properties. In this study, we have evaluated the differential proteomic responses of a potential novel probiotic Pediococcus pentosaceus M41 under heat, cold, acid, and bile stress conditions. We identified stress response proteins that could provide tolerances against these stresses and could be used as probiotic markers for evaluating stress tolerance. Pediococcus pentosaceus M41 was exposed for 2 h to each condition: 50°C (heat stress), 4°C (cold stress), pH 3.0 (acid stress) and 0.05% bile (bile stress). Proteomic analysis was carried out using 2D-IEF SDS PAGE and LC-MS/MS. Out of 60 identified proteins, 14 upregulated and 6 downregulated proteins were common among all the stress conditions. These proteins were involved in different biological functions such as translation-related proteins, carbohydrate metabolism (phosphoenolpyruvate phosphotransferase), histidine biosynthesis (imidazole glycerol phosphate synthase) and cell wall synthesis (tyrosine-protein kinase CapB). Proteins such as polysaccharide deacetylase, lactate oxidase, transcription repressor NrdR, dihydroxyacetone kinase were upregulated under three out of the four stress conditions. The differential expression of these proteins might be responsible for tolerance and protection of P. pentosaceus M41 against different stress conditions. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Baig, Turner, Liu, Al-Nabulsi, Shah and Ayyash.)
Databáze:	MEDLINE
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