| Autor: |
Takezawa S; Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan., Maezawa M; Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan., Tsuzuku S; Ibaraki Prefectural Kenpoku Livestock Hygiene Service Center, 966-1 Nakagachicho, Mito, Ibaraki 310-0002, Japan., Kawakami J; Ibaraki Prefectural Kenpoku Livestock Hygiene Service Center, 966-1 Nakagachicho, Mito, Ibaraki 310-0002, Japan., Oouci Y; Ibaraki Prefectural Kenpoku Livestock Hygiene Service Center, 966-1 Nakagachicho, Mito, Ibaraki 310-0002, Japan., Inokuma H; Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. |
| Abstrakt: |
Peripheral B-lymphocyte clonality of 274 bovine leukemia virus-infected cattle with lymphocytosis was analyzed using clonality PCR based on sequences of the variable region of the bovine immunoglobulin H chain. None of the cattle showed monoclonal proliferation, while 10, 31, and 233 showed minor-clonal, oligoclonal, and polyclonal proliferation, respectively. A total of 163 cattle were analyzable the following year, and lymphocytosis was maintained in 157, indicating persistent lymphocytosis (PL). B-lymphocyte clonality of the 157 PL cattle was minor-clonal in 6 (3.8%), oligoclonal in 8 (5.1%), and polyclonal in 143 (91.1%). A higher rate of enzootic bovine leukosis (EBL) onset within a year was observed in PL cattle with minor-clonal (50.0% (3/6)) and oligoclonal (25.0% (2/8)) proliferation compared to those with polyclonal (5.6% (8/143)) proliferation. Minor-clonal and oligoclonal proliferation in PL cattle may be a prognosis factor for developing EBL. |
