Quantification of airborne SARS-CoV-2 genomic particles in different hospital settings.

Autor: Amato-Lourenço LF; LIM-05, Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil. luisfamato@usp.br.; Institute of Advanced Studies (IEA) Global Cities Program, University of São Paulo, São Paulo, Brazil. luisfamato@usp.br.; Faculty of Medicine, University of São Paulo, Dr. Arnaldo Avenue, 455, Room 1150, Cerqueira Cesar, São Paulo, 01246903, Brazil. luisfamato@usp.br., de Souza Xavier Costa N; LIM-05, Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil., Dantas KC; LIM-05, Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil., Lombardi SCFS; Division of Research & Transfusion Medicine, Pro-Blood Foundation/Blood Center of São Paulo, Sao Paulo, Brazil., Júnior AM; Laboratory of Medical Investigation in Pathogenesis and Targeted Therapy in OncoImmuno-Hematology (LIM-31), Department of Hematology, Hospital das Clínicas - HCFMUSP, Faculty of Medicine, University of São Paulo, São Paulo, Brazil., Lindoso JAL; Departamento de Moléstias Infecciosas e Parasitárias - Faculty of Medicine, University of São Paulo, São Paulo, Brazil.; Institute of Infectious Diseases Infectology Emilio Ribas, Sao Paulo, Brazil., Lima FG; Heart Institute (InCor), School of Medicine at Sao Paulo University, Sao Paulo, Brazil., Carvalho-Oliveira R; LIM-05, Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil., Mauad T; LIM-05, Department of Pathology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.; Institute of Advanced Studies (IEA) Global Cities Program, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Oct 28; Vol. 11 (1), pp. 21284. Date of Electronic Publication: 2021 Oct 28.
DOI: 10.1038/s41598-021-00761-1
Abstrakt: We quantified the presence of SARS-CoV-2 RNA in the air of different hospital settings and the autopsy room of the largest medical centre in Sao Paulo, Brazil. Real-time reverse-transcription PCR was used to determine the presence of the envelope protein of SARS-CoV-2 and the nucleocapsid protein genes. The E-gene was detected in 5 out of 6 samples at the ICU-COVID-19 ward and in 5 out of 7 samples at the ward-COVID-19. Similarly, in the non-dedicated facilities, the E-gene was detected in 5 out of 6 samples collected in the ICU and 4 out of 7 samples in the ward. In the necropsy room, 6 out of 7 samples were positive for the E-gene. When both wards were compared, the non-COVID ward presented a significantly higher concentration of the E-gene than in the COVID-19 ward (p = 0.003). There was no significant difference in E-gene concentration between the ICU-COVID-19 and the ICU (p = 0.548). Likewise, there was no significant difference among E-gene concentrations found in the autopsy room versus the ICUs and wards (dedicated or not) (p = 0.245). Our results show the widespread presence of aerosol contamination in different hospital units.
(© 2021. The Author(s).)
Databáze: MEDLINE
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