Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies.

Autor:	Conde JN; Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA., Mladinich M; Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA.; Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY, USA., Schutt W; Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY, USA., Mackow ER; Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA. Erich.Mackow@stonybrook.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2409, pp. 197-205.
DOI:	10.1007/978-1-0716-1879-0_13
Abstrakt:	A growing body of evidence demonstrates that endothelial cells (ECs) play a prominent role in immune-enhanced pathology seen in dengue virus (DENV) infection that might contribute to vascular permeability and hemorrhagic manifestations in severe dengue cases. However, it remains a question of whether DENV infection of ECs directly causes permeability or if extra-endothelial factors such as immune cell activation or antibody-dependent enhancement (ADE) are required. In this chapter, we detail the measurement of the transendothelial electrical resistance (TEER), a quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial monolayers and show that DENV infection of ECs does not cause endothelial permeability in vitro. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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