Isolation of Nucleoid Fraction from Mycoplasma gallisepticum Cells with Synchronized Cell Division.

Autor: Zubov AI; Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency, Moscow, Russia. alexanderzubov@icloud.com., Ladygina VG; Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency, Moscow, Russia., Galyamina MA; Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency, Moscow, Russia., Pobeguts OV; Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency, Moscow, Russia., Fisunov GY; Federal Research and Clinical Center for Physical-Chemical Medicine, Federal Medical-Biological Agency, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Oct; Vol. 171 (6), pp. 760-763. Date of Electronic Publication: 2021 Oct 27.
DOI: 10.1007/s10517-021-05311-3
Abstrakt: It is assumed that unknown mechanisms can be involved in adaptation Mycoplasma gallisepticum to unfavorable factors, one of these can be local rearrangements of the structure and spatial organization of the chromosome. To study these mechanisms, we obtained a culture of M. gallisepticum with synchronized division and isolated the nucleoid fraction from this culture by the method of mild cell lysis and centrifugation in a sucrose gradient. Liquid chromatography-mass spectrometry analysis of the proteome showed that in comparison with the cell lysate, the nucleoid fraction was enriched with DNA-binding proteins. This analysis will help to find new nucleoid-associated proteins and to study their dynamics, distribution, and their role during infection and under stress conditions.
(© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: