Study of the Dynamics of Biofilm Formation and Elastase Activity of Pseudomonas aeruginosa in the Presence of Dodecanoyl-Homoserine Lactone.

Autor: Cherepushkina VS; Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia. lisocim@mail.ru., Mironova TE; Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia.; Novosibirsk State Agrarian University, Novosibirsk, Russia., Afonyushkin VN; Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia.; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia., Koptev VY; Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia., Nefedova EV; Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia., Donchenko NA; Siberian Federal Scientific Centre of Agro-BioTechnologies, Russian Academy of Sciences, Krasnoobsk, Russia.; Novosibirsk State Agrarian University, Novosibirsk, Russia., Dimova AS; Novosibirsk State Agrarian University, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2021 Oct; Vol. 171 (6), pp. 741-744. Date of Electronic Publication: 2021 Oct 27.
DOI: 10.1007/s10517-021-05307-z
Abstrakt: We studied the effect of early accumulation of N-3-oxo-dodecanoyl-homoserine lactone on the suppression of Pseudomonas aeruginosa reproduction, biofilm formation, and elastase activity. N-3-oxo-dodecanoyl-homoserine lactone in various concentrations was added to the P. aeruginosa culture, and changes in the concentration of bacteria and the formation of biofilms were studied in dynamics. N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 25 μM, decelerated proliferation of bacterial cells during the first 6 h of culturing (p<0.05) and stimulated biofilm formation after 18 h of culturing. Elastase activity of P. aeruginosa increased significantly after addition of N-3-oxo-dodecanoyl-homoserine lactone in a concentration of 0.75 μM.
(© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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