Proteomic profiles of Zika virus-infected placentas bearing fetuses with microcephaly.

Autor: Quiñones-Vega M; Department of Biochemistry, Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil., Velásquez E; Department of Biochemistry, Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil., Sosa-Acosta P; Department of Biochemistry, Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil., Melo A; Instituto Pesquisa Professor Joaquim Amorim Neto (IPESQ), Campina Grande, Paraíba, Brazil., Garcez PP; Institute of Biomedical Science, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil., Nogueira FCS; Department of Biochemistry, Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil.; Laboratory of Proteomics (LabProt), LADETEC, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil., Domont GB; Department of Biochemistry, Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro - RJ, Brazil.
Jazyk: angličtina
Zdroj: Proteomics. Clinical applications [Proteomics Clin Appl] 2022 Jan; Vol. 16 (1), pp. e2100042. Date of Electronic Publication: 2021 Nov 12.
DOI: 10.1002/prca.202100042
Abstrakt: Purpose: Zika virus (ZIKV) transmission to the fetus during pregnancy could enable a collection of severe fetal malformations like microcephaly (MC), termed Congenital Zika Syndrome (CZS). The mechanisms involved in ZIKV transplacental transmission are not fully understood.
Experimental Design: Here we aim to identify in placental tissues the deregulated proteins associated with ZIKV-induced MC using label-free proteomics.
Results: We found proteins associated with DNA damage and gene expression inhibition up-regulated in infected placentas with no MC fetuses (Z+) compared to the control group (Ctr). Actin filament organization and the immune response were also found deregulated in the Z+ group. In ZIKV-positive placentas bearing fetuses with MC (MC+) was detected an increase in T cell activation, indicating an elevated immune response. A comparison between MC+ and Z+ groups showed a higher abundance of proteins related to endocytosis and autophagy in MC+, suggesting a higher transcytosis of vesicles with ZIKV particles across the maternal-fetal interface.
Conclusions and Clinical Relevance: Our results suggest that higher expression of integrins in MC+ might be associated with high internalization of the virus since these proteins are known as virus receptors. Similarly, an increased immune response in the placenta and higher infiltration of the virus to the fetus could contribute to the neurological malformation of the CZS.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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