Production and immobilization of β-glucanase from Aspergillus niger with its applications in bioethanol production and biocontrol of phytopathogenic fungi.

Autor: El-Shora HM; Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt. shoraem@yahoo.com., El-Sharkawy RM; Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt., Khateb AM; Department of Medical Laboratory Technology, College of Applied Medical Sciences, Taibah University, Medina, Saudi Arabia., Darwish DB; Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt. d_darwish@mans.edu.eg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Oct 25; Vol. 11 (1), pp. 21000. Date of Electronic Publication: 2021 Oct 25.
DOI: 10.1038/s41598-021-00237-2
Abstrakt: β-Glucanase has received great attention in recent years regarding their potential biotechnological applications and antifungal activities. Herein, the specific objectives of the present study were to purify, characterize and immobilize β-glucanase from Aspergillus niger using covalent binding and cross linking techniques. The evaluation of β-glucanase in hydrolysis of different lignocellulosic wastes with subsequent bioethanol production and its capability in biocontrol of pathogenic fungi was investigated. Upon nutritional bioprocessing, β-glucanase production from A. niger EG-RE (MW390925.1) preferred ammonium nitrate and CMC as the best nitrogen and carbon sources, respectively. The soluble enzyme was purified by (NH 4 ) 2 SO 4 , DEAE-Cellulose and Sephadex G 200 with 10.33-fold and specific activity of 379.1 U/mg protein. Tyrosyl, sulfhydryl, tryptophanyl and arginyl were essential residues for enzyme catalysis. The purified β-glucanase was immobilized on carrageenan and chitosan with appreciable yield. However, the cross-linked enzyme exhibited superior activity along with remarkable improved thermostability and operational stability. Remarkably, the application of the above biocatalyst proved to be a promising candidate in liberating the associate lignocellulosic reducing sugars, which was utilized for ethanol production by Saccharomyces cerevisiae. The purified β-glucanase revealed an inhibitory effect on the growth of two tested phytopathogens Fusarium oxysporum and Penicillium digitatum.
(© 2021. The Author(s).)
Databáze: MEDLINE
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