Effects of cell concentration, time of fresh storage, and cryopreservation on peripheral blood stem cells: PBSC fresh storage and cryopreservation.

Autor: Araújo AB; Centro de Processamento Celular, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil. Electronic address: abaraujo@hcpa.edu.br., Salton GD; Centro de Processamento Celular, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil. Electronic address: gsalton@hcpa.edu.br., Angeli MH; Centro de Processamento Celular, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil. Electronic address: mangeli@hcpa.edu.br., Furlan JM; Centro de Processamento Celular, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil. Electronic address: jfurlan@hcpa.edu.br., Schmalfuss T; Centro de Processamento Celular, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil. Electronic address: tschmalfuss@hcpa.edu.br., Röhsig LM; Centro de Processamento Celular, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, RS, 90035-903, Brazil. Electronic address: lrohsig@hcpa.edu.br.
Jazyk: angličtina
Zdroj: Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis [Transfus Apher Sci] 2022 Feb; Vol. 61 (1), pp. 103298. Date of Electronic Publication: 2021 Oct 21.
DOI: 10.1016/j.transci.2021.103298
Abstrakt: Introduction: Peripheral blood stem cells are widely used in autologous or allogeneic transplantation. The quality of the product directly impacts clinical outcomes, and the cell quality and/or functionality may be influenced by the storage conditions as time, temperature, total nucleated cells (TNC) concentration and cryopreservation requirement.
Objective: To verify the effects of time, cell concentration, and cryopreservation/thawing in the viability and functionality of stem cells for transplantation.
Methods: We evaluated TNC, CD45 + viable cells, CD34 + viable cells, and cell viability and functionality of 11 samples. Measurements were performed immediately and 24 h, 48 h, 72 h, and 96 h after sample collection at high and low TNC concentrations. The same parameters were also evaluated after cryopreservation and thawing of the samples.
Result: Duration of storage and TNC concentration exhibited a negative effect on cell quality (CD45 + viable cells, CD34 + viable cells and functionality). Moreover, the association of these parameters increased the negative effect on graft quality. Cryopreservation and thawing also negatively affected the collected sample regarding viable CD34 + cells (recovery 66.2 %), viable CD45 + cells (recovery 56.8 %), and 7-AAD viability. No significant losses in viable CD45 + /CD34 + cells and functionality were observed in the first 24 h in both TNC conditions.
Conclusion: These results emphasize the importance to consider carefully the storage conditions until transplantation, measuring TNC/μL until 24 h after collection (diluting the product when TNC > 300 × 10 3 /μL) and infusing fresh graft as soon as possible.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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