Development of a Dendrimeric Peptide-Based Approach for the Differentiation of Animals Vaccinated with FlagT4G against Classical Swine Fever from Infected Pigs.

Autor: Bohórquez JA; OIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, Spain., Defaus S; Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain., Rosell R; OIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, Spain.; Departament d'Acció Climàtica, Alimentació i Agenda Rural, Generalitat de Catalunya, 08007 Barcelona, Spain., Pérez-Simó M; OIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, Spain., Alberch M; OIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, Spain., Gladue DP; Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USA., Borca MV; Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture Greenport, Greenport, NY 11944, USA., Andreu D; Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, 08003 Barcelona, Spain., Ganges L; OIE Reference Laboratory for Classical Swine Fever, IRTA-CReSA, 08193 Barcelona, Spain.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2021 Oct 02; Vol. 13 (10). Date of Electronic Publication: 2021 Oct 02.
DOI: 10.3390/v13101980
Abstrakt: Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
Databáze: MEDLINE
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