| Autor: |
van Rijn PA; Department of Virology, Wageningen Bioveterinary Research (WBVR), 8200 RA Lelystad, The Netherlands.; Department of Biochemistry, Centre for Human Metabolomics, North-West University, Potchefstroom 2520, South Africa., Maris-Veldhuis MA; Department of Virology, Wageningen Bioveterinary Research (WBVR), 8200 RA Lelystad, The Netherlands., Spedicato M; Public Health Department, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', 64100 Teramo, Italy., Savini G; Public Health Department, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', 64100 Teramo, Italy., van Gennip RGP; Department of Virology, Wageningen Bioveterinary Research (WBVR), 8200 RA Lelystad, The Netherlands. |
| Abstrakt: |
Bluetongue (BT) is a midge-borne OIE-notifiable disease of ruminants caused by the bluetongue virus (BTV). There are at least 29 BTV serotypes as determined by serum neutralization tests and genetic analyses of genome segment 2 encoding serotype immunodominant VP2 protein. Large parts of the world are endemic for multiple serotypes. The most effective control measure of BT is vaccination. Conventionally live-attenuated and inactivated BT vaccines are available but have their specific pros and cons and are not DIVA compatible. The prototype Disabled Infectious Single Animal (DISA)/DIVA vaccine based on knockout of NS3/NS3a protein of live-attenuated BTV, shortly named DISA8, fulfills all criteria for modern veterinary vaccines of sheep. Recently, DISA8 with an internal in-frame deletion of 72 amino acid codons in NS3/NS3a showed a similar ideal vaccine profile in cattle. Here, the DISA/DIVA vaccine platform was applied for other serotypes, and pentavalent DISA/DIVA vaccine for "European" serotypes 1, 2, 3, 4, 8 was studied in sheep and cattle. Protection was demonstrated for two serotypes, and neutralization Ab titers indicate protection against other included serotypes. The DISA/DIVA vaccine platform is flexible in use and generates monovalent and multivalent DISA vaccines to combat specific field situations with respect to Bluetongue. |
