Reflective mirror-based line-scan adaptive optics OCT for imaging retinal structure and function.

Autor:	Pandiyan VP; Department of Ophthalmology, University of Washington School of Medicine, Seattle, WA 98109, USA.; Co-first authors with equal contribution.; vimalbme@uw.edu., Jiang X; Department of Ophthalmology, University of Washington School of Medicine, Seattle, WA 98109, USA.; Co-first authors with equal contribution., Kuchenbecker JA; Department of Ophthalmology, University of Washington School of Medicine, Seattle, WA 98109, USA., Sabesan R; Department of Ophthalmology, University of Washington School of Medicine, Seattle, WA 98109, USA.; rsabesan@uw.edu.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2021 Aug 27; Vol. 12 (9), pp. 5865-5880. Date of Electronic Publication: 2021 Aug 27 (Print Publication: 2021).
DOI:	10.1364/BOE.436337
Abstrakt:	Line-scan OCT incorporated with adaptive optics (AO) offers high resolution, speed, and sensitivity for imaging retinal structure and function in vivo . Here, we introduce its implementation with reflective mirror-based afocal telescopes, optimized for imaging light-induced retinal activity (optoretinography) and weak retinal reflections at the cellular scale. A non-planar optical design was followed based on previous recommendations with key differences specific to a line-scan geometry. The three beam paths fundamental to an OCT system -illumination/sample, detection, and reference- were modeled in Zemax optical design software to yield theoretically diffraction-limited performance over a 2.2 deg. field-of-view and 1.5 D vergence range at the eye's pupil. The performance for imaging retinal structure was exemplified by cellular-scale visualization of retinal ganglion cells, macrophages, foveal cones, and rods in human observers. The performance for functional imaging was exemplified by resolving the light-evoked optical changes in foveal cone photoreceptors where the spatial resolution was sufficient for cone spectral classification at an eccentricity 0.3 deg. from the foveal center. This enabled the first in vivo demonstration of reduced S-cone (short-wavelength cone) density in the human foveola, thus far observed only in ex vivo histological preparations. Together, the feasibility for high resolution imaging of retinal structure and function demonstrated here holds significant potential for basic science and translational applications. Competing Interests: VPP and RS have a commercial interest in a US patent describing the technology for the line-scan OCT for optoretinography (© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)
Databáze:	MEDLINE
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