Moisture adsorption by decellularized bovine pericardium collagen matrices studied by terahertz pulsed spectroscopy and solid immersion microscopy.

Autor:	Musina GR; Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia.; Bauman Moscow State Technical University, Russia.; guzel-musina12@mail.ru., Chernomyrdin NV; Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia.; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia.; World-Class Research Center 'Digital Biodesign & Personalized Healthcare', Sechenov First Moscow State Medical University (Sechenov University), Russia., Gafarova ER; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia.; World-Class Research Center 'Digital Biodesign & Personalized Healthcare', Sechenov First Moscow State Medical University (Sechenov University), Russia., Gavdush AA; Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia.; Bauman Moscow State Technical University, Russia., Shpichka AJ; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia.; World-Class Research Center 'Digital Biodesign & Personalized Healthcare', Sechenov First Moscow State Medical University (Sechenov University), Russia.; Chemistry Department, Lomonosov Moscow State University, Russia., Komandin GA; Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia.; Bauman Moscow State Technical University, Russia., Anzin VB; Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia., Grebenik EA; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia., Kravchik MV; Scientific Research Institute of Eye Diseases, Russia., Istranova EV; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia., Dolganova IN; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia.; World-Class Research Center 'Digital Biodesign & Personalized Healthcare', Sechenov First Moscow State Medical University (Sechenov University), Russia.; Institute of Solid State Physics of the Russian Academy of Sciences, Russia., Zaytsev KI; Prokhorov General Physics Institute of the Russian Academy of Sciences, Russia.; Bauman Moscow State Technical University, Russia.; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia.; kirzay@gmail.com., Timashev PS; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), Russia.; World-Class Research Center 'Digital Biodesign & Personalized Healthcare', Sechenov First Moscow State Medical University (Sechenov University), Russia.; Chemistry Department, Lomonosov Moscow State University, Russia.; Department of Polymers and Composites, N. N. Semenov Institute of Chemical Physics, Russia.; timashev_p_s@staff.sechenov.ru.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2021 Aug 02; Vol. 12 (9), pp. 5368-5386. Date of Electronic Publication: 2021 Aug 02 (Print Publication: 2021).
DOI:	10.1364/BOE.433216
Abstrakt:	In this paper, terahertz (THz) pulsed spectroscopy and solid immersion microscopy were applied to study interactions between water vapor and tissue scaffolds-the decellularized bovine pericardium (DBP) collagen matrices, in intact form, cross-linked with the glutaraldehyde or treated by plasma. The water-absorbing properties of biomaterials are prognostic for future cell-mediated reactions of the recipient tissue with the scaffold. Complex dielectric permittivity of DBPs was measured in the 0.4-2.0 THz frequency range, while the samples were first dehydrated and then exposed to water vapor atmosphere with 80.0 ± 5.0% relative humidity. These THz dielectric measurements of DBPs and the results of their weighting allowed to estimate the adsorption time constants, an increase of tissue mass, as well as dispersion of these parameters. During the adsorption process, changes in the DBPs' dielectric permittivity feature an exponential character, with the typical time constant of =8-10 min, the transient process saturation at =30 min, and the tissue mass improvement by =1-3%. No statistically-relevant differences between the measured properties of the intact and treated DBPs were observed. Then, contact angles of wettability were measured for the considered DBPs using a recumbent drop method, while the observed results showed that treatments of DBP somewhat affects their surface energies, polarity, and hydrophilicity. Thus, our studies revealed that glutaraldehyde and plasma treatment overall impact the DBP-water interactions, but the resultant effects appear to be quite complex and comparable to the natural variability of the tissue properties. Such a variability was attributed to the natural heterogeneity of tissues, which was confirmed by the THz microscopy data. Our findings are important for further optimization of the scaffolds' preparation and treatment technologies. They pave the way for THz technology use as a non-invasive diagnosis tool in tissue engineering and regenerative medicine. Competing Interests: The authors declare no conflict of interest. (© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)
Databáze:	MEDLINE
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