Tracking the Evolutionary Trends Among Small-Size Fishes of the Genus Pyrrhulina (Characiforme, Lebiasinidae): New Insights From a Molecular Cytogenetic Perspective.
| Autor: | de Moraes RLR; Laboratorio de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, Brazil., Sassi FMC; Laboratorio de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, Brazil., Bertollo LAC; Laboratorio de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, Brazil., Marinho MMF; Museu de Zoologia da Universidade de São Paulo (MZUSP), São Paulo, Brazil.; Laboratório de Sistemática e Morfologia de Peixes, Departamento de Sistemática e Ecologia (DSE), Universidade Federal da Paraíba (UFPB), João Pessoa, Brazil., Viana PF; Laboratório de Gentética Animal, Instituto Nacional de Pesquisa da Amazônia, Coordenação de Biodiversidade, Manaus, Brazil., Feldberg E; Laboratório de Gentética Animal, Instituto Nacional de Pesquisa da Amazônia, Coordenação de Biodiversidade, Manaus, Brazil., Oliveira VCS; Laboratorio de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, Brazil., Deon GA; Laboratorio de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, Brazil.; Laboratório de Biologia Cromossômica, Estrutura e Função, Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, Brazil., Al-Rikabi ABH; Institute of Human Genetics, University Hospital Jena, Jena, Germany., Liehr T; Institute of Human Genetics, University Hospital Jena, Jena, Germany., Cioffi MB; Laboratorio de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in genetics [Front Genet] 2021 Oct 06; Vol. 12, pp. 769984. Date of Electronic Publication: 2021 Oct 06 (Print Publication: 2021). |
| DOI: | 10.3389/fgene.2021.769984 |
| Abstrakt: | Miniature fishes have always been a challenge for cytogenetic studies due to the difficulty in obtaining chromosomal preparations, making them virtually unexplored. An example of this scenario relies on members of the family Lebiasinidae which include miniature to medium-sized, poorly known species, until very recently. The present study is part of undergoing major cytogenetic advances seeking to elucidate the evolutionary history of lebiasinids. Aiming to examine the karyotype diversification more deeply in Pyrrhulina , here we combined classical and molecular cytogenetic analyses, including Giemsa staining, C-banding, repetitive DNA mapping, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) to perform the first analyses in five Pyrrhulina species ( Pyrrhulina aff. marilynae , Pyrrhulina sp., P. obermulleri , P. marilynae and Pyrrhulina cf. laeta ). The diploid number (2n) ranged from 40 to 42 chromosomes among all analyzed species, but P. marilynae is strikingly differentiated by having 2n = 32 chromosomes and a karyotype composed of large meta/submetacentric chromosomes, whose plesiomorphic status is discussed. The distribution of microsatellites does not markedly differ among species, but the number and position of the rDNA sites underwent significant changes among them. Interspecific comparative genome hybridization (CGH) found a moderate divergence in the repetitive DNA content among the species' genomes. Noteworthy, the WCP reinforced our previous hypothesis on the origin of the X Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 de Moraes, Sassi, Bertollo, Marinho, Viana, Feldberg, Oliveira, Deon, Al-Rikabi, Liehr and Cioffi.) |
| Databáze: | MEDLINE |
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