Similarities and Differences in the Acute-Phase Response to SARS-CoV-2 in Rhesus Macaques and African Green Monkeys.

Autor:	Coleman C; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, United States., Doyle-Meyers LA; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Medicine, Tulane University School of Medicine, New Orleans, LA, United States., Russell-Lodrigue KE; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Medicine, Tulane University School of Medicine, New Orleans, LA, United States., Golden N; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States., Threeton B; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States., Song K; Center for Translational Research in Infection and Inflammation, Department of Pediatrics and Department of Medicine, Tulane University School of Medicine, New Orleans, LA, United States., Pierre G; Center for Translational Research in Infection and Inflammation, Department of Pediatrics and Department of Medicine, Tulane University School of Medicine, New Orleans, LA, United States., Baribault C; Center for Research & Scientific Computing, Tulane University Information Technology, New Orleans, LA, United States., Bohm RP; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Medicine, Tulane University School of Medicine, New Orleans, LA, United States., Maness NJ; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, United States., Kolls JK; Center for Translational Research in Infection and Inflammation, Department of Pediatrics and Department of Medicine, Tulane University School of Medicine, New Orleans, LA, United States., Rappaport J; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, United States., Mudd JC; Department of Immunology and Microbiology, Tulane National Primate Research Center, Covington, LA, United States.; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2021 Oct 06; Vol. 12, pp. 754642. Date of Electronic Publication: 2021 Oct 06 (Print Publication: 2021).
DOI:	10.3389/fimmu.2021.754642
Abstrakt:	Understanding SARS-CoV-2 immune pathology is critical for the development of effective vaccines and treatments. Here, we employed unbiased serial whole-blood transcriptome profiling by weighted gene network correlation analysis (WGCNA) at pre-specified timepoints of infection to understand SARS-CoV-2-related immune alterations in a cohort of rhesus macaques (RMs) and African green monkeys (AGMs) presenting with varying degrees of pulmonary pathology. We found that the bulk of transcriptional changes occurred at day 3 post-infection and normalized to pre-infection levels by 3 weeks. There was evidence of coordination of transcriptional networks in blood (defined by WGCNA) and the nasopharyngeal SARS-CoV-2 burden as well as the absolute monocyte count. Pathway analysis of gene modules revealed prominent regulation of type I and type II interferon stimulated genes (ISGs) in both RMs and AGMs, with the latter species exhibiting a greater breadth of ISG upregulation. Notably, pathways relating to neutrophil degranulation were enriched in blood of SARS-CoV-2 infected AGMs, but not RMs. Our results elude to hallmark similarities as well as differences in the RM and AGM acute response to SARS-CoV-2 infection, and may help guide the selection of particular NHP species in modeling aspects of COVID-19 disease outcome. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Coleman, Doyle-Meyers, Russell-Lodrigue, Golden, Threeton, Song, Pierre, Baribault, Bohm, Maness, Kolls, Rappaport and Mudd.)
Databáze:	MEDLINE
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