Genotoxic impact of aluminum-containing nanomaterials in human intestinal and hepatic cells.

Autor: Jalili P; ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, 10B rue C. Bourgelat, 35306 Fougères, France., Huet S; ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, 10B rue C. Bourgelat, 35306 Fougères, France., Burel A; MRic Cell Imaging Platform, BIOSIT, University of Rennes 1, campus Santé de Villejean, 2 avenue du Pr Léon Bernard - CS, 34317, 35043 Rennes, France., Krause BC; Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany., Fontana C; INRS, 1, rue du Morvan - CS 60027, 54519 Vandoeuvre les Nancy, France., Chevance S; Université de Rennes 1, CNRS, ISCR (Institut des Sciences Chimiques de Rennes)-UMR6226, F-35000 Rennes, France., Gauffre F; Université de Rennes 1, CNRS, ISCR (Institut des Sciences Chimiques de Rennes)-UMR6226, F-35000 Rennes, France., Guichard Y; INRS, 1, rue du Morvan - CS 60027, 54519 Vandoeuvre les Nancy, France., Lampen A; Federal Institute for Risk Assessment (BfR), Max-Dohrn-Straße 8-10, 10589 Berlin, Germany., Laux P; Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany., Luch A; Federal Institute for Risk Assessment (BfR), Department of Chemical and Product Safety, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany., Hogeveen K; ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, 10B rue C. Bourgelat, 35306 Fougères, France., Fessard V; ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Toxicology of Contaminants Unit, 10B rue C. Bourgelat, 35306 Fougères, France. Electronic address: Valerie.fessard@anses.fr.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2022 Feb; Vol. 78, pp. 105257. Date of Electronic Publication: 2021 Oct 22.
DOI: 10.1016/j.tiv.2021.105257
Abstrakt: Exposure of consumers to aluminum-containing nanomaterials (Al NMs) is an area of concern for public health agencies. As the available data on the genotoxicity of Al 2 O 3 and Al 0 NMs are inconclusive or rare, the present study investigated their in vitro genotoxic potential in intestinal and liver cell models, and compared with the ionic form AlCl 3 . Intestinal Caco-2 and hepatic HepaRG cells were exposed to Al 0 and Al 2 O 3 NMs (0.03 to 80 μg/cm 2 ). Cytotoxicity, oxidative stress and apoptosis were measured using High Content Analysis. Genotoxicity was investigated through γH2AX labelling, the alkaline comet and micronucleus assays. Moreover, oxidative DNA damage and carcinogenic properties were assessed using the Fpg-modified comet assay and the cell transforming assay in Bhas 42 cells respectively. The three forms of Al did not induce chromosomal damage. However, although no production of oxidative stress was detected, Al 2 O 3 NMs induced oxidative DNA damage in Caco-2 cells but not likely related to ion release in the cell media. Considerable DNA damage was observed with Al 0 NMs in both cell lines in the comet assay, likely due to interference with these NMs. No genotoxic effects were observed with AlCl 3 . None of the Al compounds induced cytotoxicity, apoptosis, γH2AX or cell transformation.
(Copyright © 2021. Published by Elsevier Ltd.)
Databáze: MEDLINE
Externí odkaz: